| 丰白散对COPD模型大鼠肺组织中MMP-9及TIMP-1表达的影响 |
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| 引用本文： | 王煜,苏南湘. 丰白散对COPD模型大鼠肺组织中MMP-9及TIMP-1表达的影响[J]. 疑难病杂志, 2011, 10(4): 285-287,327,288. DOI: 10.3969/j.issn.1671-6450.2011.04.017 |
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| 作者姓名： | 王煜 苏南湘 |
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| 作者单位： | 1. 中南大学湘雅二医院,长沙,410011
2. 中南大学湘雅二医院中医科 |
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| 摘 要： | 目的观察丰白散对慢性阻塞性肺疾病(COPD)模型大鼠肺组织中基质金属蛋白酶-9(MMP-9)及基质金属蛋白酶组织抑制剂-1(TIMP-1)基因表达的影响,探讨其治疗COPD的作用机制。方法取10只大鼠为正常组,不予任何处理,另取大鼠36只采用烟熏加气管内滴加脂多糖(LPS)方法复制COPD模型,取造模成功的30只大鼠随机分为模型组、沐舒坦组和丰白散组各10只,予相应干预28天后,用苏木素—伊红(HE)染色评估各组大鼠肺组织病理改变,逆转录聚合酶链式反应(RT-PCR)检测MMP-9、TIMP-1 mRNA表达水平。结果正常组肺泡结构完整,肺泡间隔无缺损;模型组肺泡结构破坏、融合、弹力纤维网破坏;沐舒坦组、丰白散组病理改变程度明显减轻。模型组大鼠肺组织MMP-9、TIMP-1 mRNA表达水平明显高于正常组(P<0.05);沐舒坦组、丰白散组大鼠肺组织MMP-9、TIMP-1 mRNA表达水平较模型组下降(P<0.05),2组间差异无统计学意义(P>0.05)。结论 MMP-9、TIMP-1过度表达,MMP-9/TIMP-1失衡在COPD气道重构中发挥重要作用;丰白散能下调MMP-9、TIMP-1 mRNA表达,调节MMP-9/TIMP-1失衡,干预气道重构。
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| 关 键 词： | 丰白散 肺疾病,阻塞性,慢性 基质金属蛋白酶-9 基质金属蛋白酶组织抑制剂-1 大鼠 |
Effect of Fengbaisan on the expression of MMP-9 and TIMP-1 in rats with chronic obstructive pulmonary disease |
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| WANG Yu,SU Nan-xiang. Effect of Fengbaisan on the expression of MMP-9 and TIMP-1 in rats with chronic obstructive pulmonary disease[J]. Journal of Difficult and Complicated Cases, 2011, 10(4): 285-287,327,288. DOI: 10.3969/j.issn.1671-6450.2011.04.017 |
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| Authors: | WANG Yu SU Nan-xiang |
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| Affiliation: | WANG Yu~*,SU Nan-xiang.* The Second Xiangya Hospital Affiliated Central South University,Hunan Province,Changsha 410011,China |
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| Abstract: | Objective To observe the effect of the traditional Chinese medicine Fengbaisan on the expression of matrix metalloproteinase-9(MMP-9)and tissue inhibitor of metalloproteinase-1(TIMP-1 ) in rats with chronic obstructive pulmonary disease(COPD),and to investigate the potential mechanisms of the decoction.Methods There were 10 rats enrolled as control group,with another 36 rats induced with COPD model,which was established by modified method of combining fumigation and lipopolysaccharide(LPS) intra tracheal dr... |
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| Keywords: | Fengbaisan decoction Pulmonary disease obstructive chronic Matrix metalloproteinase-9 Tissue inhibitor of metalloproteinase-1 Rats |
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