败毒饮的薄层鉴别与含量测定

目的：建立败毒饮的质量标准。方法采用薄层色谱法对该制剂中赤芍、黄芩进行定性鉴别，采用 HPLC 同时对主要成分黄芩苷、蒙花苷进行定量测定。结果薄层鉴别色谱斑点清晰，分离度好，对照药材或对照品与阴性样品相比，在相应位置上无相同颜色斑点，阴性无干扰；黄芩苷和蒙花苷分别在0.2179～2.1790μg(r2＝0.9999)、0.1319～1.3190μg(r2＝0.9997)范围内线性关系良好。黄芩苷和蒙花苷加样回收率的RSD值分别为1.51%、2.01%。结论此方法简单方便，准确度及重复性好，可用于败毒饮的质量控制方法。

Study on the TLC and quantitative determination by UV-HPLC forBaiduyinsyrup

Objective To establish the quality control ofBaiduyin syrup.Methods The TLC was used to identity Radix paeoniae rubra, Radix Scutellariae. The quantitative determination of baicalin and buddleoside was completed by HPLC.Results The spots on TLC plates were distinct and high resolution. Compared with the negative samples, the contrast medicinal materials or control products showed that there were no spots of the same color in the corresponding position. The linear ranges of baicalin and buddleoside were 0.2179-2.1790 μg (r2=0.999 9), 0.1319-1.3190 μg (r2=0.999 7). TheRSD were 1.51% and 2.01%. Conclusions The established quality control method is simple, accurate and reproducible, which can be used for the quality control ofBaiduyin syrup.