L-精氨酸对人肾系膜细胞增殖的影响

目的　探讨L arg对人肾系膜细胞增殖的影响及其作用机制。方法　分别运用MTT法及细胞免疫组化、流式细胞仪等技术研究L arg对人肾系膜细胞增殖的影响。 结果　L arg抑制人系膜细胞增殖呈浓度、时间依赖性 ;L arg组系膜细胞PCNA表达增加 ,但细胞绝对数下降 ;流式细胞仪检测显示 ,L arg干预后大量的细胞阻滞在S期 ,L arg的浓度越高 ,阻滞作用越明显。结论　L arg对人系膜细胞增殖具有抑制作用 ,其机制可能与抑制细胞有丝分裂有关。

Effects of L-arginine on the proliferation of human renal mesangial cells

AIM To investigate the effects of L arginine on the proliferation of human renal mesangial cells and its possible mechanism in vitro . METHODS MTT assay, cells immunohistochemical detection of expression of proliferative cell nuclear antigen (PCNA) and flow cytometrical analysis were used to evaluate the influence of L arginine on the cell proliferation. RESULTS L arginine induced dose and time dependent inhibition of human mesangial cell lines (HMCL), which was partially recovered by removing of L arginine from the media. The percentage of PCNA positive cells was significantly increased in L arginine treated group, though the absolute cell number was markedly decreased. Flow cytometrical analysis showed that cells in S and G 2 M phases were significantly increased in L arginine treated group with the increasing of doses compared with those in control group. CONCLUSION L arginine may inhibit the proliferation of human mesangial cells, which is possibly related with its inhibition on cell mitogenesis.