钙离子选择性微电极在钙制剂药代动力学研究中的应用

目的 用自制的钙离子选择性微电极（Ca^2 -ISME）测定游离钙，拟反映体内钙被吸收的真实水平。方法 建立了血清样品中钙离子测定方法，并进行三种具有代表意义的站钙剂的大鼠药代动力学对比研究。经灌胃给药，定时取血，制备血清并测定其钙离子浓度。结果 三种制剂在血中的达峰时间不完全一致，但其AUC间经t检验无显著性差异。结论 C^2 -ISME可用于钙制剂的药物代谢动力学研究。

Application of Ca(2+)-ISME in the studies on pharmacokinetics of calcium supplements]

OBJECTIVE: To determined the concentration of Ca2+ in serum by a self-made Ca(2+)-ISME in order to indicate the absorption of calcium supplement. METHODS: We have developed a calibration curve method to measure Ca2+ activities in serum sample. The pharmacokinetics of three kinds of calcium supplements were investigated by the Ca(2+)-ISME after P.O. administration in 5 healthy rats. RESULTS: Though the Tmax (hours) of the three kinds of preparations were different, there were no significant differences among their AUC by paired t-text. CONCLUSION: The above results demonstrate that our self-made Ca(2+)-ISME is applicable to the researches on pharmacokinetics of calcium supplements.