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| PIK3CA基因在肝细胞癌的表达和突变 | |
| 引用本文： | 杨定华,符兆胤,黄毓,曾麒燕,周杰.PIK3CA基因在肝细胞癌的表达和突变[J].南方医科大学学报,2009,29(12):2517-2522. |
| 作者姓名： | 杨定华 符兆胤 黄毓 曾麒燕 周杰 |
| 作者单位： | 1. 南方医科大学南方医院,肝胆外科,广东,广州,510515 2. 广西医科大学,第一附属医院肝胆外科,广西,南宁,530021 3. 南方医科大学南方医院,检验科,广东,广州,510515 4. 广西医科大学,生物化学与分子生物学教研室,广西,南宁,530021 |
| 摘 要： | 目的检测PIK3CA基因在广西HCC里的表达和突变。方法收集61例手术切除,并经病理证实的肝细胞癌的癌组织及其相应的癌旁组织。用PCR-SSCP法和DNA序列测序对PIK3CA基因的第1、9和20外显子进行检测。另外,用免疫组化SP法检测癌组织和相应癌旁组织里PIK3CA基因的表达。结果PCR-SSCP法和DNA序列测序在肝癌和癌旁组织PIK3CA基因的第1、9和20外显子都没有发现PIK3CA基因点突变,但在25例癌组织第9外显子的序列峰图的末端发现有异常重叠的波峰。用免疫组化SP法检测发现50.81%癌组织里存在PIK3CA基因的高表达。结论广西HCC可能存在PIK3CA基因突变,但点突变率较低。PIK3CA在广西肝细胞性肝癌的癌组织里存在表达,其在癌组织的表达水平可能与原发性肝癌发生有关。 |
| 关 键 词： | 基因突变 肝细胞性肝癌 PIK3CA基因 |
Expression and mutations of PIK3CA gene in hepatocellular carcinomas |
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| YANG Ding-hua,FU Zhao-yin,HUANG Yu,ZENG Qi-yan,ZHOU Jie.Expression and mutations of PIK3CA gene in hepatocellular carcinomas[J].Journal of Southern Medical University,2009,29(12):2517-2522. | |
| Authors: | YANG Ding-hua FU Zhao-yin HUANG Yu ZENG Qi-yan ZHOU Jie |
| Institution: | YANG Ding-hua1,FU Zhao-yin2,HUANG Yu3,ZENG Qi-yan4,ZHOU Jie1 Department of Hepatobilliary Surgery1,Clinical Laboratory3,Nanfang Hospital,Southern Medical University,Guangzhou 510515,China,Department of Hepatobilliary Surgery2,Department of Biochemistry and Molecular Biology4,Guangxi Medical University,Nanning 530021 |
| Abstract: | Objective To investigate the expression and mutation of PIK3CA gene in hepatocellular carcinomas (HCC). Methods HCC samples and the corresponding adjacent tissues were collected from the surgical patients with pathologically verified diagnosis. The exons 1, 9 and 20 of PIK3CA gene were detected by PCR-SSCP and DNA sequencing. Immnohistochemistry was employed to test the expression of PIK3CA gene in these samples. Results No mutation was found in exons 1, 9 or 20 of PIK3CA gene in the HCC tissue and the adja... |
| Keywords: | gene mutation hepatocellular carcinoma PIK3CA gene |
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