CXCR4 RNA干扰序列筛选及对结肠癌细胞SW480生长的影响

目的筛选细胞表面趋化因子受体（CXCR4） RNA干扰高效序列,探讨其对SW480细胞生长的影响。方法采用RT-PCR和Western blot方法选择最有效的CXCR4 RNA干扰序列,流式细胞术分析转染该序列后SW480细胞生长情况。结果 sihCXCR4-3序列可使CXCR4 RNA相对含量仅为SW480组的5.4%,CXCR4蛋白相对表达量为18.95%。sihCXCR4-3组细胞数[（0.92±0.06）×105]低于SW480细胞组[（1.38±0.04）×105,P=0.0050]及NCsihCXCR4组[（1.28±0.05）×105,P=0.0256];相对SW480组和NCsihCXCR4组,sihCXCR4-3组的抑制率分别为33.03%及28.00%。sihCXCR4-3组G1期百分率明显低于SW480细胞组[（59.5±0.57）%vs.（74.11±0.54）%,P=0.0167];S期百分率高于SW480组[（23.85±0.24）%vs.（14.62±0.46）%,P=0.0305]和NCsihRNA组[（17.63±0.48）%,P=0.0013]。结论 sihCXCR4-3序列可高效沉默CXCR4基因表达,抑制SW480细胞生长。

Influence of CXCR4 RNA interference for growth of colon carcinoma cell strain SW480

Objective To select an effective CXCR4 RNA interference sequence and investigate its influence to the growth of SW480. Methods RT-PCR and Western blot were employed to find out the ideal RNA interference sequence, flow cytometry was used to analyze the growth and cell cycle of SW480. Results The relative expression of CXCR4 gene and protein in sihCXCR4-3 group was 5.4% and 18.95%, resprectively. The mean of SW480 cell was lower in sihCXCR4-3 group [(0.92 ± 0.06) × 105] than in SW480 group[(1.38 ± 0.04) × 105, P = 0.0050] and NCsihCXCR4 group [(1.28 ± 0.05) × 105, P = 0.0256]. Comparing with SW480 and NCsihCXCR4 groups,the inhibion rate of sihCXCR4-3 group was 33.03% and 28.00%, respectively. G2 stage percentage in sihCXCR4-3 group was obviously lower than in SW480 group[(59.5 ± 0.57)% vs.(74.11 ± 0.54)%,P = 0.0167], while S stage percentage was higher than in SW480 group [(23.85 ± 0.24)% vs. (14.62 ± 0.46)%, P = 0.0305] and in NCsihRNA group[(17.63 ± 0.48)%, P = 0.0013]. Conclusion sihCXCR4-3 inhibits effectively the expression of CXCR4 gene and cell proliferation of SW480.