Roux-en-Y胃转流术对2型糖尿病大鼠血清vaspin表达的影响

目的研究Roux-en-Y胃转流术(RYGB)对2型糖尿病(T2DM)大鼠的治疗作用,并探讨内脏脂肪组织产生的丝氨酸蛋白酶抑制剂(vaspin)在RYGB治疗T2DM机制中的可能作用。方法取造模成功的T2DM大鼠20只和周龄、性别相匹配的正常SD大鼠20只,用随机数字表法将其随机分为T2DM-RYGB组、T2DM-假手术组及RYGB组、假手术组,每组10只。分别于术前及术后第4和8周检测各组大鼠的空腹血糖(FPG)、血清胰岛素(INS)、血清vaspin水平及胰岛素抵抗指数(HOMA-IR),并分析血清vaspin水平与FPG、INS及HOMA-IR的相关性。结果手术前,T2DM-RYGB组与T2DM-假手术组比较以及RYGB组与假手术组比较,FPG水平、INS水平、vaspin水平及HOMA-IR差异均无统计学意义(P>0.05);而T2DM-RYGB组及T2DM-假手术组的FPG水平、INS水平、vaspin水平及HOMA-IR均分别明显高于RYGB组(P<0.05)及假手术组(P<0.05)。术后第4周,T2DM-RYGB组FPG水平、INS水平、vaspin水平及HOMA-IR较术前下降,除FPG水平(P<0.05)外,其余指标与术前比较差异均无统计学意义(P>0.05);术后第8周,FPG水平、INS水平、vaspin水平及HOMA-IR进一步下降,与术前比较差异均有统计学意义(P<0.05)。T2DM-假手术组、RYGB组及假手术组组内术前及术后第4周、第8周FPG水平、INS水平、vaspin水平及HOMA-IR比较,差异均无统计学意义(P>0.05)。手术前及手术后第4周、第8周T2DM-RYGB组与T2DM-假手术组血清vaspin水平与其对应血清INS水平、HOMA-IR均呈正相关(P<0.05)。结论 RYGB对T2DM大鼠具有一定的治疗作用,RYGB后vaspin表达水平降低,胰岛素敏感性改善,这可能是RYGB治疗T2DM的机制之一。

Role of Vaspin in Roux-en-Y Gastric Bypass on Type 2 Diabetes Mellitus Rats

Objective To study the therapeutic effect of Roux-en-Y gastric bypass(RYGB) on type 2 diabetes mellitus(T2DM) rats and explore the possible mechanism of vaspin in RYGB on T2DM.MethodsTwenty SD rats with T2DM and 20 age-and sex-matched normal SD rats were randomly divided into 4 groups according to the random digits table:T2DM-RYGB group,T2DM-sham operation(SO) group,RYGB group,and SO group,10 rats in each group.Fasting plasma glucose(FPG) level,serum insulin(INS) level,vaspin level,and homeostasis model of insulin resistance(HOMA-IR) were determined before operation and on week 4,8 after operation,respectively.At the same time,the correlation between vaspin and the indicators(FPG,INS,or HOMA-IR) was analyzed.ResultsCompared the indicators after operation with before operation,the FPG level,INS level,vaspin level,and HOMA-IR were not significantly different between the T2DM-RYGB group and T2DM-SO group(P>0.05) or between the RYGB group and SO group(P>0.05),but the FPG level,INS level,vaspin level,and HOMA-IR in the T2DM-RYGB group and T2DM-SO group were significantly higher than those in the RYGB group(P<0.05) and SO group(P<0.05),respectively.On week 4 after operation,the FPG level,INS level,vaspin level,and HOMA-IR decreased in the T2DM-RYGB group,except for the FPG level,the other indexes had no significant differences as compared with the values before operation.On week 8 after operation,the FPG level,INS level,vaspin level,and HOMA-IR further decreased in the T2DM-RYGB group,there were significant differences of these indicators between before operation and on week 8 after operation.Compared the indicators after operation with before operation,the FPG level,INS level,vaspin level,and HOMA-IR were not statistically significant(P>0.05) in the T2DM-SO group,RYGB group,or SO group.The changes in serum vaspin level correlated positively with those in INS and HOMA-IR before operaion and on week 4,8 after operaion in the T2DM-RYGB group and T2DM-SO group rats(P<0.05),respectively.ConclusionsRYGB surgery has a therapeutic effect on T2DM rats,and serum vaspin level decreases and insulin resistance is improved after RYGB surgery,which may be one of the mechanisms of the treatment for T2DM.