病毒性心肌炎外周血淋巴细胞凋亡与坏死的临床与实验研究

为探讨病毒性心肌炎患儿外周血淋巴细胞凋亡与坏死的关系及其临床意义，采用AnnexinV／PI双参数技术在流式细胞仪上定量检测了20例病毒性心肌炎患儿和40只CVB

Apoptosis and necrosis of peripheral blood lymphocytes of patients with viral myocarditis

To investigate the relationship and clinical significance between apoptosis and necrosis of peripheral blood lymphocytes in children and murine models with viral myocarditis.Annexin/PI was used to measure quantitatively the percentage of apoptosis and necrosis in peripheral blood lymphocytes by using flow cytometry.The results showed that the percentage of apoptosis and necrosis in peripheral blood lymphocytes was much higher in children with viral myocarditis than that in normal control children(P<0.001),and there was a positive correlation between the percentage of apoptosis and necrosis(r=0.81,P<0.01).The similar results were observed in murine myocarditis.Both the percentage of apoptosis and necrosis correlated positively with myocardial histopathological score(P<0.001).The increasing of lymphocytes apoptosis and necrosis in peripheral blood resulted in the decrease of cell immunologic function.The percentage of apoptosis and necrosis in peripheral blood lymphocytes can be used to estimate the severity of myocardial histopathology.