单纯疱疹病毒Ⅰ型体外感染人口腔上皮细胞的实验研究

目的研究单纯疱疹病毒Ⅰ型(HSV-1)体外感染人口腔上皮细胞的途径和方式。方法在体外利用非洲绿猴肾细胞大量扩增获得HSV-1,将此病毒体外感染人口腔上皮细胞,建立HSV-1体外感染人口腔上皮细胞模型,并收集HSV-1感染人口腔上皮细胞后的上清液转移感染Vero细胞。利用倒置显微镜对病毒感染进行形态学鉴定。应用聚合酶链式反应技术(PCR)对病毒核酸进行检测。结果倒置显微镜下人口腔上皮细胞未观察到典型的细胞病变,上清液转移感染后的Vero细胞发生典型细胞病变。利用PCR法可在单纯疱疹病毒Ⅰ型感染后的人口腔上皮细胞内检测到病毒核酸。结论HSV-1可直接感染人口腔上皮细胞。

Experimental study on herpes simplex virus type 1 infection of human oral epithelial cell in vitro

Objective To explore the approaches and methods of herpes simplex virus type 1(HSV-1)infecting human oral epithelial cell in vitro which is similar to the infection in vivo.Methods HSV-1 strains were abundantly amplified in Vero cells and then infected human oral epithelial cells.The culture supernatant was collected to infect Vero cell.Morphology of HSV-1 was identified by inverted microscope.Nucleic acid of the virus was detected by PCR.Results The infected human oral epithelial cells didn' t display obvious cytopathic effects(CPE)under inverted microscope,while Vero cells infected by the culture supernatant showed typical CPE.The nucleic acid of HSV-1 could be detected in infected human oral epithelial cell by PCR.Conclusion HSV-1 could successfully infect human oral epithelial cells.The model may provide a useful approach for studying the pathogenesis of herpes virus-associated periodontal diseases.