TLR2- and 4-independent immunomodulatory effect of high molecular weight components from Ascaris suum |
| |
Authors: | Bruna C Favoretto Sandriana R Silva Jacqueline F Jacysyn Niels OS Câmara Eliana L Faquim-Mauro |
| |
Institution: | 1. Laboratory of Immunopathology, Butantan Institute, São Paulo, SP, Brazil;2. Pasteur Institute, São Paulo, Brazil;3. LIM62 Faculty of Medicine, University of São Paulo, São Paulo, Brazil;4. Department of Immunology, University of São Paulo, São Paulo, Brazil |
| |
Abstract: | Components of high molecular-weight (PI) obtained from Ascaris suum extract down-regulate the Th1/Th2-related immune responses induced by ovalbumin (OVA)-immunization in mice. Furthermore, the PI down-modulates the ability of dendritic cells (DCs) to activate T lymphocytes by an IL-10-mediated mechanism. Here, we evaluated the role of toll like receptors 2 and 4 (TLR2 and 4) in the modulatory effect of PI on OVA-specific immune response and the PI interference on DC full activation. An inhibition of OVA-specific cellular and humoral responses were observed in wild type (WT) or in deficient in TLR2 (TLR2−/−) or 4 (TLR4−/−) mice immunized with OVA plus PI when compared with OVA-immunized mice. Low expression of class II MHC, CD40, CD80 and CD86 molecules was observed in lymph node (LN) cells from WT, TLR2−/− or TLR4−/− mice immunized with OVA plus PI compared with OVA-primed cells. We also verified that PI was able to modulate the activation of DCs derived from bone marrow of WT, TLR2−/− or TLR4−/− mice induced in vitro by agonists of TLRs, as observed by a decreased expression of class II MHC and costimulatory molecules and by low secretion of pro-inflammatory cytokines. Its effect was accompanied by IL-10 synthesis. In this sense, the modulatory effect of PI on specific-immune response and DC activation is independent of TLR2 or TLR4. |
| |
Keywords: | PI High molecular weight components from Ascaris suum Asc Ascaris suum extract DC Dendritic cell APC antigen presenting cell TLR2 toll like receptor 2 TLR4 toll like receptor 4 CLR C-type lectin receptor DTH delayed-type hypersensitivity reaction ELISA enzyme linked immunosorbent assay |
本文献已被 ScienceDirect 等数据库收录! |
|