局部涂抹木犀草素抑制小鼠干燥性皮肤瘙痒

目的：对木犀草素对小鼠干燥性皮炎的作用进行了评价。方法：用丙酮和乙醚的混合液(1：1)局部敷于ICR小鼠皮肤造成干燥性皮炎模型，造模期间，小鼠皮肤局部应用木犀草素(2，20，100μg／site)，5天后观察45min内小鼠的自发瘙瘁行为，检测造模处皮肤的PGE2含量。此外，我们还对木犀草素体外对compound 48／80(50μg／ml)引起的大鼠腹腔肥大细胞脱颗粒情况进行了评价。结果：木犀草素能够显著减少干燥性皮炎引起的小鼠的自主瘙痒行为，降低病变皮肤中的PGE2含量，并能有效抑制compound 48／80(50μg／ml)引起的大鼠腹腔肥大细胞脱颗粒。结论：木犀草素能有效减轻小鼠干燥性皮炎的瘙痒症状，其作用可能是通过对肥大细胞脱颗粒及局部炎症的抑制来实现的。

Topical Application of Luteolin Inhibits Dry Skin Pruritus in Mice

AIM:To investigate the effects of luteolin on dry skin pruritus in mice.METHOD:Prepare the dry skin prurtius model in ICR mice with cutaneous application of acetone/diethylether(1∶1) and luteolin was locally painted on the treated place(2,20,100 μg/site). Spontaneous scratching times were counted for 45 minutes,and the PGE2 content in the treated place was measured after scratching observation.We still investigated the effects of luteolin on the degranulation of mast cells induced by compound 48/80(50 μg/ml)in vitro.RESULT:Luteolin significantly inhibited the times of scratching behavior associated with dry skin pruritus,decreased the level of PGE2 located in the treated skin and blocked the degranulation of mast cells evoked by compound 48/80 in vitro. Meanwhile,histological examination demonstrated that luteolin can reduce the infiltration of lymphocytes and inflammatory cells and ameliorate the histopathological changes in the dermis.CONCLUTION:Luteolin can reduce the spontaneous scratching behavior associated with dry skin pruritus in mice and its effects against pruritus are maybe contributed to its inhibition on mast cell activation and anti-inflammatory activities.