瘢痕疙瘩成纤维细胞差异蛋白的初步分析

目的:筛选瘢痕疙瘩差异表达蛋白,为临床诊断提供实验依据。方法:以瘢痕疙瘩为实验组,正常皮肤组织为对照组,同时提取两组蛋白质,经初步消化、过柱、洗脱、收集后,加样到WCX2蛋白质芯片上,蛋白质芯片采用蛋白质芯片阅读机(PBSII-C型)读取数据。结果:实验结果表明瘢痕疙瘩组织相比较正常皮肤组织,蛋白芯片捕获262个蛋白峰。其中有33个蛋白质在瘢痕疙瘩成纤维细胞中出现明显的变化,有11个标志蛋白在瘢痕疙瘩成纤维细胞中高表达,22个标志蛋白相对正常皮肤是低表达。结论:运用SELDI分析了瘢痕疙瘩差异蛋白质,这些差异蛋白可为将来确定瘢痕疙瘩诊断标志物提供依据。

Analysis of differential display protein between keloid and normal skin

Objective Analysis the different protein between keloid and normal skin,provide a trace for diagnosis of the keloid.Methods The sample of keloid is the test group,and the normal skin is the control group.Extract the protein of two group samples respectively,digest and transfer the protein to a spin-column,wash and collect samples.Add the protein sample to WCX2 protein chip,the results read by PBSII-C apparatus.Results The protein chip capture 262 protein peak values,and 33 protein peak values are different between keloid and normal skin.11 protein peak values are high in keloid tissues,and 22 protein peak values are lower.Conclusion Using the technology of SELDI,we find several differential protein of between keloid and normal skin tissue.