Detection of antibodies to HLA1 antigens by enzyme immunoassay |
| |
| Authors: | S. D. Borozdenkova A. Yu. Baryshnikov T. N. Palkina F. S. Baranova |
| |
| Affiliation: | (1) Department of Immunology, Research Institute of Transplantology and Artificial Organs, Ministry of Health of Russia, Moscow;(2) Department of Experimental Diagnosis of Tumors, Cancer Research Center, Russian Academy of Medical Sciences, Moscow |
| |
| Abstract: | A modification of the MAILA (monoclonal antibody specific immobilization of lymphocyte antigens) method has been developed for the detection of antibodies to class 1 histocompatibility antigens. Russian biotin-treated monoclonal antibodies IKO-53 (Medbiospektr, Moscow) were used. In a complex with monoclonal antibodies, lymphocyte HLA antigen was found to retain its antigenic properties when stored for a long time. High specificity and sensitivity of the method were demonstrated. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 120, N o 9, pp. 315–317, September, 1995 Presented by V. I. Shumakov, Member of the Russian Academy of Medical Sciences |
| |
| Keywords: | antibodies to class 1 histocompatibility antigens ELISA |
| 本文献已被 SpringerLink 等数据库收录！ |
|
