增生性玻璃体视网膜病变组织中的单核细胞趋化因子-1的意义

目的：增生性玻璃体视网膜病变（PVR）是多种细胞和细胞因子参与的一种眼内创伤修复反应。单核细胞趋化蛋白-1（MCP-1）对巨噬细胞和淋巴细胞有特异趋化作用。本研究目的在于了解视网膜脱离后不同时期的组织中MCP-1的表达与巨噬细胞和淋巴细胞的关系。方法：选用视网膜手术取材标本，包括孔源性视网膜脱离的视网膜下液中的细胞成份，B级PVR玻璃体手术集取物以及PVR C/D的视网膜增殖膜，免疫组化法检测其中MCP-1，CD68和CD3表达状态。结果：视网膜下液中细胞成份MCP-1的表达均为阴性或弱阳性，B级PVR手术集取物以及增殖膜中，MCP-1表达阳性程度随PVR等级的升高而逐渐增高。CD68阳性细胞（巨噬细胞）与CD3阳性细胞（T-淋巴细胞）的比例随PVR病变程度的增加而降低。结论：MCP-1对视网膜脱离后的愈合过程和PVR增殖膜的形成有调节作用。

Significance of monocyte chemotactic protein-1 in specimens of proliferative vitreoretinopathy

AIM To investigate the relationship between the presence of MCP 1 and macrophages and T lymphocytes in specimens obtained from retinal detachment and PVR eyes. METHODS Specimens were obtained during retinal surgery, including cellular components of subretinal fluids in rhegmatogenous retinal detachment (RD), fluids collections during vitrectomy for PVR grade B, and epiretinal membranes from PVR grade C or D. Immunohistochemical detection was performed to examine the distribution of MCP 1, and the presence of CD 68 or CD 3 positive cells in these specimens. RESULTS The expression of MCP 1 was negative or very weak in cells of subretinal fluid from RD. As a function of the increase of PVR grade, MCP 1 expression became stronger while the rate of CD 68 (+)/CD 3 (+) decreased. CONCLUSION MCP 1 may modulate the wound healing after retinal detachment and the formation of epiretinal membranes in PVR.