在流动血液中蜂胶对血小板活性的抑制作用

目的： 探讨蜂胶提取液对流动状态下血小板活性的影响。方法: 采用体外灌注法，用纤维蛋白原和人Ⅲ型胶原蛋白包被盖玻片，复制血管内膜创伤模型，正常血液流经纤维蛋白原和胶原膜表面后，在切应力为 1 000/s 时，测量血小板粘附率。浓度为0.1 g/L的24%乙醇蜂胶提取液作实验组，加入24%的乙醇作阴性对照组，同浓度的阿魏酸乙醇溶液作阳性对照组。结果: 分别以纤维蛋白原和胶原蛋白膜模拟创伤表面，两实验组血小板粘附率均明显低于阴性对照组，P<0.01；而实验组与阳性对照组比较，均无明显差异，P>0.05。当血液标本流经两个不同的蛋白膜表面时，蜂胶提取液对血小板在纤维蛋白原膜表面粘附率的抑制作用更明显，P<0.01。结论： 蜂胶乙醇提取液能抑制血小板的活化，降低其在纤维蛋白原和胶原蛋白膜表面的粘附活性，对血小板与纤维蛋白原的作用更明显。

Inhibitory effects of propolis on platelet adhesion in flowing blood in vitro

AIM:To investigate effects of propolis-ethanol extract on platelet activity in flowing blood. METHODS:Intima-injury model was made by in vitro perfusion. Coverslips was coated by using fibrinogen and human Ⅲ type collagen to detect platelet adhesive rate when blood went through fibrinogen and collagen surface in 1 000/s shear rate. Three experimental groups were set up: negative control group (24% ethanol), experimental group (0.1 g/L, 24% propolis-ethanol extract) and positive control group (0.1 g/L, ferulic acid). RESULTS:Intima-injury model was made. On fibrinogen and collagen, platelet adhesion on area of experimental group was lower than that in negative control group (P<0.01). There was no significant difference between experimental group and positive control group (P>0.05). Propolis-ethanol extract decreased more obviously the platelet adhesion area on fibrinogen surface than that in collagen surface (P<0.01). CONCLUSION:Propolis-ethanol extract inhibits platelet activation and reduces its adhesion on fibrinogen and collagen surface, especially in fibrinogen surface.