PXMP4促进结直肠癌细胞增殖、迁移和侵袭

目的 探讨过氧化物酶体膜蛋白4(peroxisomal membrane protein 4,PXMP4)对结直肠癌细胞增殖、迁移和侵袭能力的影响.方法 采用生物信息学分析65例配对结直肠癌组织中PXMP4 mRNA的表达.应用免疫组化检测112例配对结直肠癌组织中PXMP4的表达,并进行临床病理相关分析;利用RT-P...

PXMP4 promotes proliferation,migration and invasion of colorectal cancer cells

Purpose To determine the expression of PXMP4 in colorectal cancer and the effects of PXMP4 on the proliferation,migration and invasion of colorectal cancer cells.Methods Bioinformatics methods were used to analyze the expression of PXMP4 mRNA in 65 paired colorectal cancer and normal tissues.The expression of PXMP4 in 112 matched colorectal cancer tissues was detected by immunohistochemistry,and the clinicopathological correlation was analyzed.The expression of PXMP4 in up-down expression cell lines were verified by RT-PCR and western blot.CCK-8,scratch healing assay and Transwell test were used to detect the effects of PXMP4 on the proliferation,migration and invasion of colorectal cancer cell lines.Results The relative expression of PXMP4 mRNA in 64 cases(total 65 cases)of colorectal cancer tissues was higher than that in normal intestinal mucosa tissues,and the difference was statistically significant(P<0.05).The positive rate of PXMP4 in 112 cases of colorectal cancer was 71.429%(80/112),which was higher than 5.4%(6/112)in normal intestinal mucosa.According to the degree of tumor differentiation and lymph node metastasis,the positive rate of PXMP4 was significantly different(P<0.05).Compared with empty vector group and negative control group,over-expression of PXMP4 can significantly promote the proliferation,migration and invasion of colorectal cancer cells(P<0.05).Interfering with PXMP4 can significantly inhibit the proliferation,migration and invasion of colorectal cancer cells(P<0.05).Conclusion The expression of PXMP4 is significantly upregulated in colorectal cancer tissues,which is related to the degree of tumor differentiation and lymph node metastasis.PXMP4 promotes the proliferation,migration and invasion of colorectal cancer cells.