miR-205和miR-944在宫颈癌患者血清中的表达及其临床意义

目的探讨宫颈癌患者血清miR-205和miR-944的表达及其临床意义。方法选取2017年1月~2019年12月本院收治的162例宫颈癌患者(宫颈癌组)、85例宫颈上皮内瘤变(CIN)患者(CIN组)和65例健康体检女性(对照组),检测各组血清miR-205及miR-944的表达水平,分析其与临床病理特征的关系。应用ROC曲线分析血清miR-205及miR-944对宫颈癌的诊断价值。应用Pearson相关分析检验血清miR-205与miR-944表达水平的相关性。结果宫颈癌组血清miR-205(1.25±0.61,0.71±0.13,0.14±0.10)及miR-944(0.82±0.38,0.46±0.02,0.03±0.01)水平均明显高于CIN组和对照组(P<0.01)。宫颈癌患者血清miR-205及miR-944表达水平与临床分期、浸润深度及淋巴结转移有关(P<0.05)。ROC曲线分析显示,血清miR-205及miR-944诊断宫颈癌的最佳截断值分别为0.94、0.53,两项联合诊断宫颈癌的曲线下面积最大(0.926,95%CI:0.864~0.985),其敏感度和特异度为92.0%和88.7%。相关分析显示,宫颈癌患者血清miR-205与miR-944的表达水平呈正相关(r=0.824,P<0.01)。结论血清miR-205及miR-944的表达水平在宫颈癌患者中明显升高,两项联合检测有助于指导宫颈癌的诊断。

Expression Levels and Clinical Significance of Serum miR-205 and miR-944 in Cervical Cancer Patients

Objective To investigate the expression levels and clinical significance of serum miR-205 and miR-944 in patients diagnosed with cervical cancer.Methods One hundred sixty two patients with cervical cancer(cervical cancer group),85 patients with cervical intraepithelial neoplasia(CIN group)and 65 healthy women(control group)in our hospital were selected from January 2017 to December 2019.The expression levels of serum miR-205 and miR-944 in each group were detected,and the relationship between expression levels and clinicopathological characteristics was analyzed.The diagnostic value of miR-205 and miR-944 in cervical cancer was analyzed using receiver operating characteristic(ROC)curve.Pearson correlation analysis was used to analyze the correlation between miR-205 and miR-944.Results The serum levels of miR-205(1.25±0.61 vs 0.71±0.13,0.14±0.10)and miR-944(0.82±0.38 vs 0.46±0.02,0.03±0.01)in cervical cancer group were significantly higher than those in CIN group and control group(P<0.01).In cervical cancer patients,the expression levels of miR-205 and miR-944 were correlated to clinical stage,depth of invasion and lymph node metastasis(P<0.05).The ROC curves showed that the optimal cut-off values of miR-205 and miR-944 for diagnostic tests of cervical cancer were 0.94 and 0.53,respectively.The area under the curve(AUC)of the two combined diagnosis of cervical cancer was the largest(0.926,95%CI:0.864-0.985),with 92.0%sensitivity,88.7%specificity.The correlation analysis showed that there was a positive relationship between miR-205 and miR-944 in cervical cancer patients(r=0.824,P<0.01).Conclusion The expression levels of miR-205 and miR-944 in cervical cancer patients were significantly higher,and a combination of the two tests can help guide the diagnosis of cervical cancer.