| 抗IL-5抗体联合抗IL-13抗体对支气管哮喘小鼠气道炎症及气道高反应性的影响 |
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| 引用本文： | 魏亚强,王丽丽,赵红,吴朔,李志奎. 抗IL-5抗体联合抗IL-13抗体对支气管哮喘小鼠气道炎症及气道高反应性的影响[J]. 中华哮喘杂志(电子版), 2010, 4(6): 321-324 |
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| 作者姓名： | 魏亚强 王丽丽 赵红 吴朔 李志奎 |
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| 作者单位： | 1. 第四军医大学西京医院呼吸内科,西安,710032
2. 解放军537医院内二科,宝鸡,710600 |
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| 基金项目： | 国家自然科学基金资助项目(30770926) |
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| 摘 要： | 目的 观察抗IL-5抗体（Anti-IL-5 Ab）联合抗IL-13抗体（Anti-IL-13 Ab）对支气管哮喘（简称哮喘）小鼠气道炎症及气道高反应性的影响,为临床应用奠定基础.方法 50只雌性Balb/c小鼠随机分成正常组、哮喘组、Anti-IL-5 Ab治疗组、Anti-IL-13 Ab治疗组和Anti-IL-5 Ab联合Anti-IL-13Ab治疗组.以清洁级Balb/c小鼠构建哮喘模型,采用腹腔注射卵蛋白和氢氧化铝混悬液处理动物.采用瑞氏染色对哮喘小鼠进行支气管肺泡灌洗液中的细胞分类及计数,应用小鼠肺功能仪检测气道阻力的变化.结果 ①Anti-IL-5 Ab联合Anti-IL-13 Ab 治疗组小鼠症状较哮喘组及其他治疗组明显减轻;②Anti-IL-5 Ab联合Anti-IL-13 Ab 治疗组可有效降低哮喘小鼠支气管肺泡灌洗液中嗜酸粒细胞、中性粒细胞、淋巴细胞等炎症细胞水平,与Anti-IL-5 Ab治疗组及Anti-IL-13 Ab治疗组比较差异有统计学意义（P〈0.05）;③Anti-IL-5 Ab联合Anti-IL-13 Ab治疗组可有效降低哮喘小鼠气道高反应性,与Anti-IL-5 Ab治疗组及Anti-IL-13 Ab治疗组比较差异有统计学意义（P〈0.05）.结论 Anti-IL-5 Ab 联合Anti-IL-13 Ab治疗组不但可抑制哮喘小鼠肺部嗜酸粒细胞、中性粒细胞、淋巴细胞等炎症细胞浸润,还可以抑制哮喘迟发反应,降低气道高反应性,改善肺功能,为治疗哮喘提供新的解决办法.
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| 关 键 词： | 抗IL-5抗体联合抗IL-13抗体 哮喘 气道炎症 气道高反应性 |
Effects of anti-IL-5 antibody combined anti-IL-13 antibody suppresses airway inflammation and airway hyperresponsiveness in a rat asthmatic model |
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| WEI Ya-qiang,WANG Li-li,ZHAO Hong,WU Shuo,LI Zhi-kui. Effects of anti-IL-5 antibody combined anti-IL-13 antibody suppresses airway inflammation and airway hyperresponsiveness in a rat asthmatic model[J]. Chinese Journal of Asthma(Electronic Version), 2010, 4(6): 321-324 |
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| Authors: | WEI Ya-qiang WANG Li-li ZHAO Hong WU Shuo LI Zhi-kui |
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| Affiliation: | WEI Ya-qiang,WANG Li-li,ZHAO Hong,WU Shuo,LI Zhi-kui.Department of Respiratory Diseases,Xijing Hospital,the Forth Military Medical University,Xi'an 710032,China |
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| Abstract: | Objective The effects of airway inflammation and airway hyperresponsiveness(AHR)were observed by using anti-IL-5 antibody combined anti-IL-13 antibody in a rat asthmatic model,which was established a foundation for clinical application.Methods Fifty female Balb/c rats were divided randomly into 5 groups:the normal control group,the asthmatic model group,the anti-IL-5 antibody group,the anti-IL-13 antibody group and anti-IL-5 antibody combined anti-IL-13 antibody group.A rat asthmatic model was established.T... |
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| Keywords: | Anti-IL-5 antibody combined anti-IL-13 antibody Asthma Airway inflammation Airway hyperresponsiveness |
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