| 苦瓜籽MAP30的聚乙二醇化学修饰条件优化及纯化研究 |
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| 引用本文： | 张永明,刘盛邦,黄凯,聂宇,孟延发. 苦瓜籽MAP30的聚乙二醇化学修饰条件优化及纯化研究[J]. 中草药, 2008, 39(11): 1640-1643 |
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| 作者姓名： | 张永明 刘盛邦 黄凯 聂宇 孟延发 |
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| 作者单位： | 四川大学生命科学学院,生物资源与生态环境教育部重点实验室,四川,成都,610064 |
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| 基金项目： | 国家自然科学基金 |
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| 摘 要： | ![]()
目的探讨聚乙二醇修饰苦瓜籽核糖体失活蛋白(MAP30)的反应条件以及修饰产物的纯化方法。方法在不同条件下,将聚乙二醇活性酯(mPEG)2-NHS与MAP30反应,用SDS-PAGE分析不同反应条件对产物成分的影响;采用Sephacryl S-300HR分子筛柱色谱法对修饰产物进行分离纯化,TNBS法测定目标产物PEG-MAP30的氨基修饰度。结果聚乙二醇修饰MAP30的最优反应条件为4℃,pH8.5硼酸硼砂缓冲液,MAP30质量浓度1mg/mL,MAP30与(mPEG)2-NHS质量比1∶10为最佳修饰条件,5min内反应完全;反应产物进一步纯化,经SDS-PAGE检测目标产物PEG-MAP30中不含未修饰的MAP30;TNBS法测定MAP30的氨基修饰度为44.9%。结论初步确定了聚乙二醇修饰MAP30的反应条件和修饰产物的纯化方法。
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| 关 键 词： | 苦瓜籽 核糖体失活蛋白(MAP30) 聚乙二醇 化学修饰 |
| 收稿时间： | 2008-04-07 |
Modification conditions optimization and purification of PEGylated MAP30 from Semen Momordicae Charantiae |
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| ZHANG Yong-ming,LIU Sheng-bang,HUANG Kai,NIE Yu and MENG Yan-fa. Modification conditions optimization and purification of PEGylated MAP30 from Semen Momordicae Charantiae[J]. Chinese Traditional and Herbal Drugs, 2008, 39(11): 1640-1643 |
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| Authors: | ZHANG Yong-ming LIU Sheng-bang HUANG Kai NIE Yu MENG Yan-fa |
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| Affiliation: | Key Laboratory of Bio-resources and Eco-environment, Ministry of Education, College of Life Science, Sichuan University, Chengdu 610064, China;Key Laboratory of Bio-resources and Eco-environment, Ministry of Education, College of Life Science, Sichuan University, Chengdu 610064, China;Key Laboratory of Bio-resources and Eco-environment, Ministry of Education, College of Life Science, Sichuan University, Chengdu 610064, China;Key Laboratory of Bio-resources and Eco-environment, Ministry of Education, College of Life Science, Sichuan University, Chengdu 610064, China;Key Laboratory of Bio-resources and Eco-environment, Ministry of Education, College of Life Science, Sichuan University, Chengdu 610064, China |
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| Abstract: | ![]()
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| Keywords: | Semen Momordicae Charantiae momordica anti-HIV protein of 30 kDa(MAP30) polyethylene glycol (PEG) chemical modification |
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