非小细胞肺癌胸水细胞块c-M ET基因扩增检测分析

目的：探讨非小细胞肺癌胸水细胞块在间充质上皮转化因子受体(c-mesenchymal-epithelial transition，c-MET)基因扩增检测中的临床价值。方法：采用RT-PCR法检测215例非小细胞肺癌细胞块和404例非小细胞肺癌组织块中c-MET基因扩增，并检测细胞块同时送检组织块的患者74例的一致性。结果：细胞块c-MET基因扩增31例，扩增率14.42%(31/215)；组织块c-MET基因扩增35例，扩增率8.66%(35/404)；74例有组织块对照的细胞块c-MET结果一致性有68例，一致率达91.89%(68/74)，其中细胞块c-MET基因扩增率12.16%(9/74)，组织块扩增率17.57%(13/74)。结论：非小细胞肺癌胸水细胞块c-MET的扩增率略高于组织块；有恶性胸水的非小细胞肺癌患者原发灶组织发生c-MET扩增的概率较高。

Analysis of cell blocksc-MET amplification in pleural effusion of non-small cell lung cancer

Objective: To investigate the clinical value of the cell blocks for gene ampliifcation detection of c-mesenchymal-epithelial transition (c-MET).Methods: Two hundred and iftfeen cases of cell block from pleural effusion of non-small cell lung cancer (NSCLC) were collected. Four hundred and four cases of tissue block were detected by RT-PCR method. hTe consistency ofc-MET ampliifcation was examined in 74 cases of patients with tissue block and cell block.Results:c-MET ampliifcation was found in 31 of 215 cell blocks (positive detection rate of 14.42%). c-MET ampliifcation was detected in 35 of 404 tissue blocks (positive detection rate of 8.66%). hTere were 68 cases in the 74 (91.89%) cases had the same consistency as tissue block.c-MET ampliifcation was detected in 9 of 74 (12.16%) cell blocks, and 13 of 74 (17.57%) tissue blocks.Conclusion: hTe rate ofc-MET ampliifcation in cell blocks of non-small cell lung cancer is higher than in matched tissue blocks. hTe patients with malignant pleural effusion are likely to tendc-MET ampliifcation.