基于1型糖尿病大鼠模型的呼吸丙酮与血糖及血β-羟基丁酸相关性研究

目的 1型糖尿病（T1D）和某些2型糖尿病患者经常患有酮症（高血酮）,呼吸丙酮是糖尿病的一种潜在生物标志物.本研究使用T1D大鼠模型研究呼吸丙酮与血糖（BG）及血β-羟基丁酸（BHB）的关系.方法 使用基于光腔衰荡光谱技术的呼吸丙酮分析仪测定了20只T1D大鼠和18只健康大鼠的呼吸气体丙酮浓度,并同时测定了其BG和血液BHB浓度.结果 与健康大鼠组相比,T1D大鼠组的呼吸丙酮、BG和血液BHB间的差异均具有统计学意义（P＜0.05）.T1D大鼠和健康大鼠的呼吸丙酮与血液BHB呈显著正相关性,而与BG呈显著负相关性;但当T1D大鼠接受胰岛素治疗且血糖浓度趋向正常值时,其呼吸丙酮和血糖浓度的关系由负相关变为正相关.健康大鼠的呼吸丙酮浓度与血糖浓度则无相关性.多元线性回归分析显示,T1D大鼠的呼吸丙酮可用于其血液BHB的预测,使用10只健康大鼠和10只T1D大鼠对该回归模型进行了验证.结论 使用大鼠动物模型进行呼吸气体研究是可行的,其有助于解决人类呼吸分析研究中的定量关系问题.

Correlations of breath acetone with blood glucose and blood beta-hydroxybutyrate using type 1 diabetic rat model

Objective The breath acetone is a potential biomarker for diabetic diagnosis,because some of type 2 and type 1 diabetic (T1D) patients frequently experience ketosis.This paper aims to study the correlations of breath acetone with blood glucose(BG) and blood beta-hydroxybutyrate(BHB) using T1D rat model.Methods Breath acetone values from the 20 T1D and 18 healthy rats were determined using a cavity ringdown spectroscopy based breath analyzer.Simultaneous BG and blood BHB levels were also measured.Results The results showed that breath acetone,BG,and blood BHB in the T1D rat group all have significant difference with that in the healthy rat group (P＜0.05).A significant positive relationship between breath acetone and blood BHB was found to exist in both the T1D and healthy rats,and a significant negative relationship between breath acetone and BG was found in the T1D rats.However,the relationship between breath acetone and BG shifts from negative to weakly positive when T1D rats were treated by insulin.No correlation between breath acetone and BG was found in the healthy rats.The multiple linear regression analysis revealed that breath acetone had the predictive nature for blood BHB in T1D rats,and the predictable ability of the model was verified by testing the breath acetone and simultaneous blood BHB from additional 10 healthy rats and 10 T1D rats.Conclusions The findings of this study suggest the usage of rat model is feasible and may help address the question of quantitative correlations in human breath analysis.