| 高表达Bcl-2下调喉癌细胞黏附能力的分子机制探讨 |
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| 引用本文： | 左建宏,雷明胜,李金成,美玲,李赛,吕秀,王雷,谢金龙.高表达Bcl-2下调喉癌细胞黏附能力的分子机制探讨[J].国际病理科学与临床杂志,2016(5):540-544. |
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| 作者姓名： | 左建宏 雷明胜 李金成 美玲 李赛 吕秀 王雷 谢金龙 |
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| 作者单位： | 1. 南华大学医学院肿瘤研究所,湖南 衡阳,421001;2. 张家界市人民医院呼吸内科,湖南 张家界,427000;3. 邵阳医学高等专科学校内科学教研室,湖南 邵阳,422000;4. 解放军第一六九中心医院肿瘤科,湖南 衡阳,421002 |
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| 基金项目： | 国家自然科学基金(81272960)，湖南省科技厅重点项目资助(2013WK2010，2014SK2015)。hTis work was supported by National Nature Science Foundation of China(81272960)，Key Research Program from Science and Technology Department of Hunan Province(2013WK2010 |
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| 摘 要： | 目的：探讨高表达的Bcl-2对喉癌细胞黏附能力的影响。方法：建立并鉴定高表达Bcl-2的稳定细胞株，采用黏附实验检测细胞的黏附能力，流式细胞仪和Western blotting实验分析细胞表面integrin表达的变化。结果：成功构建高表达Bcl-2细胞株，下调了细胞的黏附能力，并降低了细胞表面 integrin α2β1的表达，而未见对integrin αv、integrin β4、integrin α3和integrin α5的表达的影响。结论：高表达Bcl-2可能通过下调了细胞表面integrin α2β1的表达从而减低细胞的黏附能力。
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| 关 键 词： | Bcl-2 黏附能力 integrin α2β1 侵袭转移 |
The investigation of molecular mechanism of the high expression of Bcl-2 down-regulating cell adhesion in laryngeal carcinoma cells |
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| Abstract: |
Objective: To investigate the effect of high expression of Bcl-2 on the cell adhesion ability.Methods: hTe stable cells of high expression of Bcl-2 were built and identiifed. hTe adhesion ability was assessed by adhesion assay. hTe expression of integrin was analyzed by FLOW and Western blotting.Results:Bcl-2 stable cells were successfully built. Bcl-2 overexpression decreased the cell adhesion and the expression of integrin α2β1, however, it did not affect the expression of integrin αv, integrin β4, integrin α3 and integrin α5.Conclusion:High expression of Bcl-2 may reduce the cell adhesion by down-regulating the expression of integrin α2β1. |
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| Keywords: | Bcl-2 adhesion ability integrin α2β1 invasion and migration |
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