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半边旗抗肿瘤有效成分5F的纯化及其体外增效作用
引用本文:兰柳波,梁念慈,莫丽儿,邓亦峰. 半边旗抗肿瘤有效成分5F的纯化及其体外增效作用[J]. 中国药理学通报, 2003, 19(7): 804-807
作者姓名:兰柳波  梁念慈  莫丽儿  邓亦峰
作者单位:广东医学院生物化学与分子生物学研究所,湛江,524023
基金项目:国家自然科学基金资助课题No 39870 99,广东省自然科学基金资助课题No 94 0 586
摘    要:
目的 寻找半边旗中二萜类有效成分之一 5F纯化的新方法 ,研究 5F对常用抗肿瘤药物的体外增效作用。方法 应用结合了AgNO3的普通层析用硅胶来纯化 5F ,应用台盼蓝拒染法测定药物对细胞的抑制率。结果 纯化后所得到的产品中 5F的含量达到 99%以上 ,是迄今为止纯度最高的产品。较低浓度的 5F分别与氟尿嘧啶 (5 Fu)、顺铂 (CD DP)及长春新碱 (VCR)合用可以增强它们对HL 60、K562细胞株的杀伤作用。结论 结合了AgNO3的普通硅胶可有效地分离纯化 5F。 5F可增强上述几种药物对HL 60、K562细胞的杀伤作用 ,可能与几种药物对细胞周期影响不同有关

关 键 词:半边旗  纯化  增效作用
文章编号:1001-1978(2003)07-0804-04
修稿时间:2003-02-07

Purification of 5F from Pteris semipinnata and its enhanced cytotoxicity in vitro
LAN Liu Bo,LIANG Nian Ci,MO Li Er,DENG Yi Feng. Purification of 5F from Pteris semipinnata and its enhanced cytotoxicity in vitro[J]. Chinese Pharmacological Bulletin, 2003, 19(7): 804-807
Authors:LAN Liu Bo  LIANG Nian Ci  MO Li Er  DENG Yi Feng
Abstract:
AIM To find a new method for purifying the active compound 5F isolated from Pteris semipinnata L.(PsL ) and observe its enhanced cytotoxicity when combined with other antitumor agents. METHODS Silica gel combined with AgNO 3 was made to purify 5F. Cytotoxicity was detected with trypan blue dye exclusion assay. RESULTS After purification, the concentration of compound 5F in purified products was higher than 99%. The inhibitory rates of 5F combined with 5 Fu or CDDP or VCR were higher than that of these drugs used alone. CONCLUSIONS Compound 5F could be purified effectively using silica gel combined with AgNO 3. 5F could enhance the cytotoxicity on HL 60 and K562 cells of the drugs mentioned above. The different effect of these agents on the various phases of cell cycle kinetics may explain the enhanced effect of 5F.
Keywords:Pteris semipinnata L  purification  cytotoxicity
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