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丹仙康骨胶囊对培养成骨细胞增殖分化的影响
引用本文:刘日光,沈冯君,易诚青,刘建湘.丹仙康骨胶囊对培养成骨细胞增殖分化的影响[J].辽宁中医杂志,2003,30(1):76-77.
作者姓名:刘日光  沈冯君  易诚青  刘建湘
作者单位:华中科技大学同济医学院协和医院骨科,湖北,武汉,430022
摘    要:目的 :为了解补肾活血中药丹仙康骨胶囊对体外培养成骨细胞的作用。方法 :应用MTT ,对硝基苯磷酸盐法及骨钙素含量放免测定法 ,观察丹仙康骨胶囊对成骨细胞增殖与分化作用的影响。结果 :丹仙康骨胶囊刺激成骨细胞增殖 ,提高ALP活性及骨钙素含量。结论 :丹仙康骨胶囊具有刺激体外成骨细胞增殖分化的功能

关 键 词:丹仙康骨胶囊  细胞培养  成骨细胞
文章编号:1000-1719(2003)01-0076-02
修稿时间:2002年9月20日

Effects of Danxian Kanggu Capsule on Proliferation and Differentiation of Cultured Osteoblasts in Vitro
LIU Ri guang,SHEN Feng jun,YI Cheng qing,LIU Jian xiang.Effects of Danxian Kanggu Capsule on Proliferation and Differentiation of Cultured Osteoblasts in Vitro[J].Liaoning Journal of Traditional Chinese Medicine,2003,30(1):76-77.
Authors:LIU Ri guang  SHEN Feng jun  YI Cheng qing  LIU Jian xiang
Abstract:Objective :To investigate the effects of Danxian Kanggu(DXKG)capsule extracted from traditional Chinese medicine on cultured osteoblasts in vitro. Methods :MTT,PNPP and BGP content radioimmunoassay were used to observe the proliferation,the activity of ALP and the BGP content of osteoblasts cultured in vitro. Results :It was found that DXKG capsule had the effects on stimulating the proliferation,ALP activity and the BGP content of cultured osteoblasts in different concentration. Conclusion :DXKG capsule has effects on stimulating the proliferation and the differentiation,and increasing the activity of ALP and the BGP content of cultured osteoblasts in vitro.
Keywords:Danxian Kanggu capsule  cell culture  osteoblast
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