淋巴组织增生性疾病组织中检测细小病毒B19的意义

目的;探讨人细小病毒Ｂ１９（ＨＰＶＢ１９）与增生性淋巴组织疾病的相关性。方法：慢性淋巴结炎伴反应性增生４２例,恶性淋疾病的相关性,方法：慢性淋巴结炎伴反应性增４２例,恶性淋巴瘤２９例,结核性淋巴结炎１５例,采用巢式ＰＣＲ技术扩增８６例石包埋活检淋巴结组织Ｂ１９－ＤＮＡ。结果：检出Ｂ１９ＤＮＡ１８例,阳性率为２０．９％,其中慢性淋为检出１５例,阳性率３５．７％;恶性淋巴瘤检出３例,阳民生率为１０．３

Detection significance of human parvovirus B19 in lymphoproliferative diseases

AIM: To study the relationship between human parvovirus B19 and lymphoproliferative diseases. METHODS: Paraffin embedded biopsy lymphadens from 84 patients with lymphoproliferative disease, including 42 patients with chronic proliferative lymphadenitises, 29 patients with malignant lymphoma and 15 patients with tuberculous lymphadenitises, were examined for the presence of human parvovirus B19 DNA by nested PCR assay technique. RESULTS: B19 DNA positive rate was 20.9% (18/86) in all specimens, including 35.7% (15/42) in chronic lymphadenitis, 10.3% (3/29) in malignant lymphoma and 0% (0/15) in tuberculous lymphadenitises. CONCLUSION: HPVB19 is one of the important causes in chronic proliferative lymphadenitis. The relationship between parvovirus B19 and malignant lymphoma need further study.