冬虫夏草菌丝体发酵液中多糖的分离纯化与含量测定

目的]从冬虫夏草菌丝体发酵液中提取,分离虫草多糖,并建立样品中多糖的含量测定方法。方法]采用D101大孔树脂分离冬虫夏草菌丝体发酵液中的多糖;采用苯酚-硫酸法测定样品中多糖含量。结果]虫草多糖的得率为14·2g/L;样品中虫草多糖的含量为70·6%。结论]方法简便,准确,重现性好,可用于冬虫夏草菌丝体发酵液中虫草多糖的提取纯化和含量测定。

Separation, Purification and Content Test of Polysaccharide in Zymotic Fluid of Cordycepts Mycelium

Objective] To extract and separate polysaccharide from Cordyceps mycelium, and establish content test method of polysaccharide in sample. Method] Take foramen magnum resin D101 to separate polysaccharide from the zymotic fluid of Cordyceps mycelium, the phenol-SO2 method to measure polysaccharide in sample. Result] The receiving rate of polysaccharide was 14.2g/L; the content was 70.6% from sample. Conclusion] The said method is simple, convenient, correct, can be used for separation, purification and content measurement of polysaccharide from the zymotic fluid of Cordyceps mycelium.