金威消石颗粒镇痛作用的实验研究

目的:探索金威消石颗粒对乙酸致腹膜炎小鼠的镇痛作用及对小鼠热板法痛阈值的影响.方法:取ICR小鼠60只随机分为金威消石颗粒低、中、高剂量组、阳性对照组和阴性对照组,每组12只,分别经胃灌注不同剂量金威消石颗粒、双氯芬酸钠溶液和蒸馏水,日1次,连续7d.各小鼠于末次给药后1h由腹腔注射0.7％乙酸溶液0.2 mL,计数注射后20 min内各小鼠的扭体次数.另取ICR小鼠60只,随机分为上述5组,以上法经胃灌注上述液体,以平板法检测各小鼠给药前及给药后1h、2h、3h和5h的痛阅值.结果:金成消石颗粒各剂量组乙酸致腹膜炎小鼠的平均扭体次数均明显低于阴性对照组(P＜0.01);金威消石颗粒各剂量组在给药后2h、3h和5h时的痛阈值均明显高于阴性对照组(P＜0.01);各剂量组3h和5h痛阈值明显高于各组给药前基础痛阈值和1h痛阈值(P＜0.01).结论:金威消石颗粒可提高ICR小鼠热板法痛阈值,减少乙酸致腹膜炎小鼠的扭体次数,具有明显的镇痛作用.

Experimental Study on Analgesic Effect of Jinwei Xiaoshi Granules

Objective: To explore the analgesic effect of Jinwei Xiaoshi Granules on mice with acetic acid-induced peritonitis and its influence on the pain threshold of mice by plate method.Methods: Sixty ICR mice were randomly divided into three pilot groups(low-,middle-and high-dose group),one positive control group and one negative control group,12 mice in each group.The corresponding mice were given stomach perfusion of respectively,once a day,for 7 days.Each mouse was injected intra-celiacally 0.2 mL of 0.7% acetic acid solution 1 hour just after the final perfusion,and the torsion numbers of all mice during postinjection twenty minutes’ period were counted.Other ICR 60 mice were randomly divided into 5 groups as mentioned above and managed by the same stomach perfusion,and plate method was used to measure the pain thresholds of mice before and 1 h,2 hrs,3 hrs and 5 hrs after stomach perfusion in each group.Results: The mean torsion numbers of mice in three pilot groups were less than those in negative control group(P < 0.01),no significant difference existed in the mean torsion numbers of mice between three pilot groups and positive control groups(P > 0.05).The mean pain thresholds of mice 2 hrs,3 hrs and 5hrs after the stomach perfusion were significantly higher in three pilot groups than those in negative control group(P < 0.01),whereas no significant difference when compared to positive control group(P > 0.05).The mean pain thresholds of mice 3 hrs and 5 hrs after stomach perfusion were significantly higher than those before and 1h after stomach perfusion(P < 0.01).Conclusions: Jinwei Xiaoshi Granules can increase the pain threshold of ICR mice by plate method,decrease the torsion numbers of mice with acetic acid-induced peritonitis,implying a considerable analgesic effect.