AlCl3 induces lymphocyte apoptosis in rats through the mitochondria–caspase dependent pathway |
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| Authors: | Miao Li Miao Song Li‐Min Ren Chun‐Yu Xiu Jian‐Yu Liu Yan‐zhu Zhu Yan‐Fei Li |
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| Affiliation: | 1. College of Veterinary Medicine, Northeast Agricultural University, Harbin, China;2. Police Dog Technical School of the Ministry of Public Security, Shenyang, China;3. Institute of Special Animal and Plant Sciences of Chinese Academy of Agricultural Sciences, Changchun, China |
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| Abstract: | To investigate apoptosis mechanisms in lymphocytes induced by aluminum trichloride (AlCl3) through the mitochondria–caspase dependent pathway, the spleen lymphocytes of rats were cultured with RPMI‐1640 medium and exposed to AlCl3·6H2O in the final concentrations of 0 (control group, CG), 0.3 (low‐dose group, LG), 0.6 (mid‐dose group, MG), and 1.2 (high‐dose group, HG) mmol·L?1 for 24 h, respectively. Mitochondrial transmembrane potential (ΔΨm), cytochrome C (Cyt C) protein expression in cytoplasm, Caspase‐3 and Caspase‐9 activity, Bcl‐2, Bax, Caspase‐3 and Caspase‐9 mRNA expressions, DNA ladder and lymphocytes apoptosis index were detected. The results showed that Cyt C protein expression in cytoplasm, Caspase‐3 and Caspase‐9 activity, Bcl‐2, Bax, Caspase‐3 and Caspase‐9 mRNA expressions, the ratio of Bcl‐2 and Bax mRNA expression, lymphocytes apoptosis index increased, while ΔΨm decreased in the AlCl3‐treated groups compared with those in CG. The results indicate that AlCl3 induces lymphocyte apoptosis in rats through the mitochondria–caspase dependent pathway. © 2014 Wiley Periodicals, Inc. Environ Toxicol 31: 385–394, 2016. |
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| Keywords: | aluminum apoptosis rat spleen lymphocytes mitochondria caspase |
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