三七总皂苷分期治疗对脑出血大鼠微管相关蛋白-2及神经生长相关蛋白表达的影响

目的探讨三七总皂苷（tPNS）分期治疗对脑出血大鼠微管相关蛋白-2（MAP-2）及神经生长相关蛋白（GAP-43）表达的影响。方法大鼠随机分为5组：正常对照组、假手术组、脑出血模型组、脑出血后12h治疗A组、脑出血后72h治疗B组。治疗A组于脑出血后12h、治疗B组于脑出血后72h开始以三七总皂苷70mg／（kg·d）腹腔注射，连用4d。其余各组皆同时注射等量生理盐水。大鼠于造模后7d、14d、21d每个时间点分别处死，干湿重法测脑舍水量，脑组织切片行MAP-2、GAP-43免疫组化。结果治疗组大鼠肢体功能较脑出血模型组好，且治疗A组神经功能评分在7d、14d、21d优于治疗B组（P〈0．05）；治疗组可明显改善脑水肿，7d时治疗A组脑含水量低于治疗B组（P〈0．05）。治疗组血肿周边MAP-2及GAP-43表达皆高于脑出血模型组（P〈0．05）；且治疗A组在7d、14d、21d3个时间点表达高于治疗B组（P〈0．05）。结论tPNS可以减轻大鼠脑出血后的脑水肿并积极上调MAP-2和GAP-43表达；早期给予tPNS治疗脑出血大鼠较晚期运用可能对大鼠康复更有利。

Effect of Total Panax Notoginseng Saponins on Expression of MAP - 2 and GAP - 43 in Brains of Rats with Intracerebral Hemorrhage

Objective To investigate the effect of total panax notoginseng saponins（tPNS）on the expression of microtubule -asso- ciated protein - 2 （MAP - 2） and growth associated protein - 43 （GAP - 43） in the brains of rats with intracerebral hemorrhage （ICH）. Methods Experimental rats were divided into five groups：Normal control group,sham operation group,ICH model group, treatment A group treated with tPNS after operation 12 h,treatment B group treated with tPNS after operation 72 h. Rat model with intracere- bral hemorrhage was duplicated by injecting collagenase into right corpus striatum （RCS）. In treatment group, tPNS was injected intraperitoneally for four days. Rats were randomly sacrificed at postoperative 7th, 14th and 21th days. Nervous function defection and refection of rats were studied by Longa EZ score. The brain water content was measured by wet - dry method. The expressing of MAP - 2 and GAP-43 were determined by immunohistoehemistry staining. Results The scores of Longa EZ in treatment A group were better than that in treatment B group （ P 〈 0. 0 5 ） . At 7 th day , there was significant difference in brain water contents between in treatment A group and in treatment B group（P〈0.05）. Compared with ICH model group,the expression of MAP - 2 and GAP - 43 were in- creased in treatment group （P〈0.05）. And at three time points,the expression of MAP -2 and GAP- 43 in treatment A group were higher than that in treatment B group（P〈0.05）. Conclusion The tPNS could effectively reduce brain edema after ICH and upregulate expression of MAP - 2 and GAP - 43. Early use of tPNS in ICH might be better than it was used at advanced stage.