血府逐瘀汤干预急性心肌缺血心肌细胞凋亡与Bcl-2、Bax表达的实验研究

目的 观察血府逐瘀汤对心肌细胞凋亡与相关蛋白表达干预的作用。方法 采用结扎冠状动脉左前降支的方法复制急性心肌缺血模型，15条犬随机分成时照组、心肌缺血组以及血府逐瘀汤组，每组5条。分别采用HE染色、DNA末端标记法（TUNEL）以及免疫组化法观察心肌组织病理变化、心肌细胞凋亡与相关蛋白表达的变化。结果 对照组无细胞变性与坏死，仅见极少量凋亡阳性细胞；心肌缺血纽有明显心肌组织坏死，心肌细胞凋亡增多，Bcl-2的表达明显减少，Bax的表达明显增多，与对照组比较有统计学意义（P〈0．05）；血府避瘀汤组心肌细胞坏死及凋亡均显著减轻，Bcl-2的表达明显增多，Bax的表达明显减少，与心肌缺血组比较有统计学意义（P〈0．05）。结论 血府逐瘀汤可影响Bcl-2和Bax的表达，有效地抑制心肌细胞坏死及凋亡，减轻心肌细胞损伤，对缺血心肌有保护作用。

Effect of Xuefuzhuyu Decoction on Myocardial Cellular Apoptosis and Expressions of Bcl - 2 and Bax in Acute Experimental Ischemic Myocardium

Objective TO explore the effect of Xuefuzhuyu decoction on myocardial cellular apoptosis and expressions of Bcl - 2 and Bax in acute experimental myocardial ischemia. Methods Fifteen dogs were randomly divided into 3 groups：Control group; ischemic group and Xuefuzhuyu group （5 each group）. The left anterior descending coronary artery was ligated to establish models of acute myocardial ischemia. The changrs in myocardial pathology,cellular apoptosis and expressions of Bcl - 2 and Bax were detected. Results Cellular degeneration and necrosis were not found in control group. Myocardium cellular apoptosis and expression of Bax were decreased,and expression of Bcl -2 was increased in xuefuzhuyu group （P〈0. 05）. Conclusion Xuefuzhuyu decoction could affect expressions of Bcl - 2 and Bax and prevent myocardial cells from apoptosis.