Ultrastructural evidence of mitotic ependymal cells in 6-aminonicotinamide-treated suckling mice

Summary Mitotic ependymal cells were encountered in 10-day-old mice treated with 6-aminonicotinamide, an antagonist of niacin. These occurred along the medial surface of the lateral ventricle and the ventral portion of the aqueduct. Electron microscopy revealed that both mitotic ependymal cells had eccentrically placed chromosomes without a nuclear membrane and well-formed gap junctions in contact with adjacent ependymal cells. Microtubules from a centriole radiated to the chromosomes. These data show that cell division occurs in morphologically matured ependymal cells in the postnatal brain under pathological conditions. We believe this to be the first ultrastructural demonstration of this phenomenon.Supported in part by research grants NS-03356, NS-10803 and training grant NS-07098 from the National Institute of Neurological and Communicative disorders and Stroke, USPHS