需行全身照射患者辐射损伤血浆代谢谱的研究

目的利用代谢组学的方法发现与人辐射损伤密切相关的特征血浆代谢物，初步探讨辐射损伤涉及的代谢通路。方法收集2012年1月至2014年5月期间40名患者全身照射（TBI）辐照前后的血浆样本，通过气相色谱-质谱（GC-MS）联用的代谢组学方法研究TBI对人血浆代谢物的影响，并筛选出与TBI损伤密切相关的特征血浆代谢物。结果 TBI后，血浆中葡萄糖、肉豆蔻酸、草酸、3-羟基丁酸、尿素、天冬氨酸、缬氨酸、亮氨酸、赖氨酸和苏氨酸含量显著增加（P<0.05），胆固醇、丙酮酸、丙酸、乳酸、丙氨酸、甘氨酸、肌醇、山梨糖酐、乙二醇和次黄嘌呤含量显著降低（P<0.05）。结论 TBI可引起人体血浆中代谢物水平显著变化，主要涉及氨基酸代谢、糖代谢、脂代谢等代谢通路。

Preliminary study on plasma metabolites of total body irradiation patients

Objective To investigate radiation-related human plasma metabolic features by using metabonomics method and to analyze relative metabolic pathway.Methods The plasma samples of 40 patients pre-and post-total body irradiation (TBI) from January 2012 to May 2014 were collected, and the effect of TBI on human plasma metabolites was studied by gas chromatography mass spectrometry (GC-MS), and the differential plasma metabolic features related to irradiation damage were screened. Results The levels of glucose, myristic acid, oxalic acid, 3-hydroxy butyric acid, urea, aspartic acid, valine, leucine, lysine and threonine in plasma were significantly(P<0.05) increased after TBI, while the levels of cholesterol, pyruvic acid, propionic acid, lactic acid, alanine, glycine, inositol, sorbitan, ethylene glycol and hypoxanthine were decreased drastically(P<0.05).Conclusions TBI could cause significant changes in the levels of human plasma metabolites including amino acid metabolism, glucose metabolism, lipid metabolism and so on.