血管内皮生长因子C的表达和微淋巴管密度与食管癌淋巴结转移和预后的关系

目的探讨食管癌组织中血管内皮生长因子C（VEGF-C）的表达和微淋巴管密度（LVD）与食管癌淋巴结转移和预后的关系,揭示食管癌淋巴转移的可能机制。方法采用免疫组化法定性检测59例食管癌组织中VEGF-C的表达,同时采用淋巴管特异性标记物血管内皮生长因子受体3（VEGFR-3）进行免疫组化染色检测淋巴管密度,以CD34标记血管内皮检测微血管密度作为对比,结合食管癌临床病理参数和预后分析。结果食管癌组织VEGF-C的阳性表达率显著高于正常组织（P=0.05）,食管癌组织淋巴管密度（31.21±11.47）较正常组织（11.71±4.70）明显增高（P〈0.01）。淋巴结转移组VEGF-C/LVD显著高于淋巴结无转移组（P〈0.01,P〈0.05）。淋巴结转移组术后2年生存率（33.42%）显著低于无淋巴结转移组（66.93%）（P〈0.05）,VEGF-C阳性组术后2年生存率（26.69%）显著低于VEGF-C阴性组（66.95%）（P〈0.01）。结论食管癌组织VEGF-C和LVD与淋巴结转移存在显著相关,肿瘤组织可能通过调控VEGF-C促进淋巴管的生成,为肿瘤的淋巴转移提供通道,进而影响食管癌患者的预后。

Expression of VEGF-C and lymphatic vessel density with tumor lymph node metastasis and prognosis of esophageal carcinoma TAN

Objective： To study the expression of vascular endothelia growth factor C （VEGF-C）and lymphatic vessel density（LVD） in carcinoma tissue and their relationship with tumor lymph node metastasis and disease prognosis and to explore the possible mechanism of lymph metastasis. Methods ： The expression of VEGF-C in tumor areas of 59 cases of esophageal carcinomas was evaluated by immunohistochemistry. And the LVD and microvessel density（MVD） were evaluated by immunohistochemistry, using polyclonal antibody for vascular endothelia growth factor receptor-3 （VEGFR-3） and monoclonal antibody for CD34 respectively. The relationship between VEGF-C/LVD and clinicopathologic features and prognosis was analyzed. Results： The rate of positive expression of VEGF-C in tumor tissue was significantly higher than that in normal esophageal mucosa（ P = 0. 05 ）. The LVD at tumor borders （31.21 ±11.47 ） was significantly higher than that in normal tissue（ 11.71 ±4.70）and the expressions of VEGF-C and LVD were correlated. The expression of VEGF-C and LVD for the group with lymph node metastases was significantly higher than that for the one without lymph node metastases（P 〈0. 01 ,P 〈0. 05 ）. For the group with lymph node metastasis, 2 years survival rate was significantly lower than that for the one without metastasis（ P 〈 0. 05 ）For the group with positive expression of VEGF-C, 2 years survival rate was significantly lower than that for the one with negative expression（ P 〈0. 01 ）. Conclusion： High LVD and VEGF-C in tumor tissue are associated with lymph node metastasis. It is likely that via lymph node metastasis, expression of VEGF-C acts on the disease prognosis.