青蒿琥酯对MDA—MB-231细胞增殖抑制作用及其机制 |
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引用本文: | 赵小波,幸天勇,吴凯南.青蒿琥酯对MDA—MB-231细胞增殖抑制作用及其机制[J].内分泌外科杂志,2011(3):155-157,175. |
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作者姓名: | 赵小波 幸天勇 吴凯南 |
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作者单位: | [1]四川省南充市川北医学院附属医院普外三科,637000 [2]重庆医科大学附属第一医院普外五科 ,400016 |
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摘 要: | 目的探讨青蒿琥酯(artesunate,ART)对乳腺癌雌激素受体阴性细胞MDA—MB-231抑制作用及其机制。方法ART处理细胞3d后,采用MTT比色法检测细胞增殖功能,观察细胞形态、结构的改变,免疫细胞化学检测Bax,nm23,Bcl-2,P21WAF1/CIP1蛋白的表达情况。结果ART作用于乳腺癌细胞MDA—MB-231后,发现其对细胞的抑制作用呈明显浓度依赖性,可导致细胞形态、结构改变;免疫细胞化学结果显示2μmol/L青蒿琥酯作用细胞72h后,药物组同细胞对照组比较,可上调Bax、nm23、P21WAF1/CIP1蛋白表达,差异有统计学意义(P〈0.05),Bcl-2蛋白质表达差异无统计学意义(P〉0.05)。结论ART有抑制MDA—MB-231细胞增殖的作用,其机制可能与上调Bax、nm23、P21WAF1/CIP1蛋白表达有关。
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关 键 词: | 青蒿琥酯 MDA—MB-231细胞 Bax nm23 P21WAF1/CIP1 Bcl-2 |
Anti-proliferative effect of artesunate on MDA-MB-231 cell and its mechanisms |
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Authors: | ZHAO Xiao-bo XING Tian-yong WU Kai-nan |
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Institution: | . Department of General Surgery, the Affiliated Hospital of North Sichuan Medical College, Nanchong 637000, China |
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Abstract: | Objective To study the effects of artesunate (ART) on estrogen receptor negative breast cancer cell line MDA-MB-231 and its mechanisms. Methods Treated with ART for 3 days, MDA-MB-231 cells proliferation was examined by MTT assay. The morphological and uhrastructural changes of MDA-MB-231 cells were observed under microscope and electronic microscope. Immunocytoehemistry was used to detect the expression of Bax, nm23, Bcl-2, and P21WAF1/CIP1. Results ART treatment led to a dose dependent inhibition of MDA-MB-231 cells. ART could change the morphology and ultrastmcture of MDA-MB-231 cells. After treatment with ART (2μmol/L) for 72 hours, immunocytochemical staining showed that the expression of Bax, nm23, and P21WAF1/CIP1 was upregulated in comparison to the control group (P 〈 0. 05) while the expression of Bcl-2 in MDA-MB-231 cells didn't have a significant change ( P 〉 0. 05 ). Conclusions ART shows an anti-proliferative effect on MDA-MB-231 cells. The mechanisms may be related to upregulation of Bax, nm23 and P21WAF1/CIPI expression. |
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Keywords: | Artesunate MDA-MB-231 cell Bax nm23 P21WAF1/CIP1 Bcl-2 |
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