白藜芦醇和乙醇对内毒素诱导小鼠腹腔巨噬细胞炎性因子产生的作用

目的:研究白藜芦醇和乙醇对细菌内毒素活化的小鼠腹腔巨噬细胞炎性因子产生的相互作用.方法:Griess试剂法检测NO产生;小鼠胸腺细胞增殖法检测IL-1的产生;ELISA检测IL-6,TNF-α的生成.结果:自藜芦醇(6.25,12.5,25 μmol/L)和乙醇(0.2％,0.8％)剂量依赖性和协同性地抑制24小时LPS(1 mg/L)和IFN-γ(5 kU/L)刺激的NO的产生;25μmol/L的白黎芦醇还抑制IL-6的产生,此作用可被乙醇加强;乙醇单独对24小时IL-1的产生无影响,但可提高自藜芦醇促进IL-1产生的作用;低剂量乙醇抑制巨噬细胞TNF-α产生,但两种浓度的乙醇与白黎芦醇共用时均增强白黎芦醇对TNF-α的产生的促进作用.结论:白黎芦醇和乙醇对巨噬细胞功能分子的产生有调控作用.

Effects of resveratrol and ethanol on production of pro-inflammatory factors from endotoxin activated murine macrophages

AIM: To study the interaction of resveratrol and ethanol on the production of pro-inflammatory factors from activated murine peritoneal macrophages (MPM). METHODS: NO production was measured with Griess assay; IL-1 production was measured through thymocyte co-stimulating assay; IL-6 and TNF-alpha were detected by ELISA method. RESULTS: Resveratrol (6.25, 12.5, 25 micromol/L) and ethanol (0.2 %, 0.8 %) synergistically inhibited the 24 h production of NO from lipopolysaccharide (LPS, 1 mg/L) and IFN-gamma (5 kU/L) stimulated MPM; resveratrol at higher dose (25 micromol/L) also inhibited IL-6 production. Ethanol additively strengthened this effect. Ethanol had no significant influence on 24 h MPM IL-1 production, but it promoted the ability of resveratrol on enhancing the IL-1 release from activated MPM. Low doses of ethanol inhibited 24 h production of TNF-alpha, however, both dose of ethanol enhanced the promoting effect of resveratrol on TNF-alpha production. CONCLUSION: Resveratrol and ethanol can interact to influence the production of macrophage function molecules, which is noteworthy in evaluating the health-care effect of wine consumption.