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二氢青蒿素对胰腺癌生长的抑制作用
引用本文:陈华,孙备,潘尚哈,李军,薛东波,孟庆辉,姜洪池. 二氢青蒿素对胰腺癌生长的抑制作用[J]. 中华外科杂志, 2004, 47(1): 1002-1005. DOI: 10.3760/cma.j.issn.0529-5815.2009.13.013
作者姓名:陈华  孙备  潘尚哈  李军  薛东波  孟庆辉  姜洪池
作者单位:哈尔滨医科大学附属第一医院肝胆胰外科,150001;
基金项目:教育部新世纪优秀人才支持计划资助项目黑龙江省杰出青年科学基金资助项目黑龙江省教育厅科学技术研究资助项目
摘    要:Objective To investigate the anti-tumor activity of dihydroartemisinin in pancreatic cancer in vitro and in vivo. Methods For cultured cells,cell growth was determined by the MTT assay and apoptosis was evaluated by flow cytometry analysis stained with Annexin V-FITG/PI. The protein expression in BxPC-3 cells was analyzed by Western blot assay. BxPC-3 cells were injected subcutaneously into nude mice to establish pancreatic xenograft tumors and the tumor volume was monitored after exposure to dihydroartemisinin. Ki-67 staining and TUNEL assay were used to assess tumor cell proliferation and apoptosis in tumor tissue. Results After treatment by dihydroartemisinin in vitro, the proliferative inhibition rates of pancreatic cancer cells BxPC-3 and AsPC-1 reached up to (76.2 ± 3.5) % and (79.5 ± 2.9) %, and the apoptosis rates were up to (55.5 ± 3.2)% and (40.0 ± 3.5)%, the differences were significantly (P < 0.01) compared with control [(2.0 ± 1.3) % and (0.9 ± 0.4) %]. Dihydroartemisinin inhibited the growth of pancreatic xenograft tumors in nude mice. The proliferation index and apoptusis index were (49.1 ± 3.9)% and (50.2 ± 4.4)% respectively in dihydroarternisinin 50 mg/kg treatment group, compared to those of (72.1 ± 3.3) % and (9.4 ± 2.9) % in control, the differences were significantly (P <0.01). Western blot assay indicated that dihydroartemisinin up-regulates expression of proliferation-associated protein p21WAF1 and down-regulates expression of PCNA, increases expression of apoptosis-associated protein Bax and decreases expression of Bcl-2 and activates caspase-9 in BxPC-3 cells. Conclusions Dihydroartemisinin exerts anti-tumor activity in pancreatic cancer both in vitro and in vivo by proliferation inhibition and apoptusis induction. Dihydroartemisinin can be used as a potential anti-tumor drug in pancreatic cancer.

关 键 词:胰腺肿瘤   二氢青蒿素   增殖   凋亡   

Study on anticancer effect of dihydroartemisinin on pancreatic cancer
Abstract:
Keywords:Pancreatic neoplasmsDihydreatermisininProliferationApoptOsis
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