单人份核酸检测在血液筛查中的应用

目的 初步了解单人份核酸检测方法在无偿献血者血液筛查中应用的利弊.方法 利用Procleix TIGRIS全自动核酸检测系统,结合血站常规的血清学检测,平行检测无偿献血者血液标本,对核酸检测初检反应性、复检非反应性的标本应用其它检测方法进行验证；对核酸检测初检反应性且鉴别阳性的部分标本,应用阴性血浆做倍比稀释,模拟混合NAT检测.结果 在总共12 005份标本中,核酸检测ULTRIO初检反应性标本共51例,经复检非反应性或鉴别试验阴性的标本共17例(33.3％),其中有6例经罗氏核酸检测为HBV阳性,即不可重复的标本存在真阳性的可能.模拟混样检测结果显示,经混样检测后阳性检出率明显降低,尤其是病毒拷贝数比较低的标本.结论 单人份核酸检测在血液筛查的应用中有利有弊,应根据需要选择.

Application of single specimen nucleic acid test in donor blood screening

Objective To study the application of nucleic acid testing of non-pooled specimens in blood screening. Methods Procleix TIGRIS automatic NAT system,together with Routine ELISA assays,was used to test blood samples.Samples with reactive results in initial screening but nonreactive in repeat tests were subjected to alternative NAT assay;NAT positive samples were diluted to simulate minipools of 4,8 and 16 NAT. Results Out of the total 12 005 samples,51 were ULTRIO reactive,and 17 were found nonreactive after repeated ULTRIO assay.And 6 of the 17 were tested HBV DNA positive by ROCHE NAT.Positive rate decreased apparently after simulating minipool NAT,especially in samples with low virus copies. Conclusion Individual donation nucleic acid test has some advantages as well as some disadvantages in blood screening,and choice should be made on the basis of the requirement.