| 肝癌高低转移细胞株差异表达基因的初步研究 |
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| 引用本文： | 吴欣,贾户亮,刘银坤,钦伦秀,余龙,李雁,陈洁,赵燕. 肝癌高低转移细胞株差异表达基因的初步研究[J]. 中国癌症杂志, 2003, 13(2): 97-101 |
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| 作者姓名： | 吴欣 贾户亮 刘银坤 钦伦秀 余龙 李雁 陈洁 赵燕 |
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| 作者单位： | 1. 复旦大学肝癌研究所,上海,200032
2. 复旦大学遗传学研究所,上海,200433 |
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| 基金项目： | 国家自然科学基金 ( 30 0 0 0 0 75)，教育部科学技术重大项目( 2 2 5A)，上海市启明星计划 ( 0 0QB14 019)，复旦大学MedX基金资助 |
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| 摘 要： | 目的：肝癌高低转移细胞株的基因表达谱的比较。方法：应用cDNA microarray技术，对肝癌高低转移细胞株的基因表达谱进行比较，并应用RT—PCR对部分差异表达的基因进行了验证。结果：肝癌高转移细胞株与低转移细胞株相比，有384个基因存在差异表达，其中12个是位于染色体8p上。选取4个基因用RT—PCR验证，结果与cDNA microarray结论基本一致。结论：肝癌高低转移细胞株基因表达存在明显差别。染色体8p上可能存在促进和抑制肝癌转移的基因。
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| 关 键 词： | 肝癌 转移 高转移细胞株 低转移细胞株 |
| 文章编号： | 1007-3639(2003)02-0097-05 |
Comparative study on gene expression difference between high and low metastatic human hepatocellular carcinoma cell strains |
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| WU Xin,JIA Hu liang,LIU Yin kun,et al. Comparative study on gene expression difference between high and low metastatic human hepatocellular carcinoma cell strains[J]. China Oncology, 2003, 13(2): 97-101 |
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| Authors: | WU Xin JIA Hu liang LIU Yin kun et al |
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| Abstract: | Purpose:To study the difference in gene expression profile between HCC cell strains with high and low metastatic potential.Methods:The gene expression profile of high and low metastatic HCC cell strains were analyzed by cDNA microarray, and genes with differential expression were further verified by RT PCR.Results:384 differentially expressed genes were detected between high and low metastatic HCC strains, making up 32% of the total genes studied. RT PCR analysis on 4 genes chosen demonstrated the same results as those revealed by the array technique.Conclusions:Obvious difference in gene expression patterns was observed between high and low metastatic HCC cell strains, chromosome 8p may contain metastasis promote and inhibitory genes. |
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| Keywords: | liver neoplasms hepatocellular carcinoma metastasis cDNA microarray chromosome 8p cultured tumor cells |
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