| RP-HPLC法测定猫豆中猫豆胍的含量 |
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| 引用本文： | 黄增琼,蒋伟哲,黄敏,黄兴振,巫世红. RP-HPLC法测定猫豆中猫豆胍的含量[J]. 中国药房, 2010, 0(11): 1000-1001 |
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| 作者姓名： | 黄增琼 蒋伟哲 黄敏 黄兴振 巫世红 |
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| 作者单位： | [1]广西医科大学药学院,南宁市530021 [2]右江民族医学院药学系,百色市533000 |
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| 基金项目： | 国家自然科学基金资助项目（30760309）;广西科学基金项目（桂科自0832014Z）;广西研究生教育创新项目（2007105981007M15） |
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| 摘 要： | 目的:建立以反相高效液相色谱(RP-HPLC)法测定猫豆中猫豆胍含量的方法。方法:色谱柱为PhenomenexTMC18(250mm×4.6mm,5μm),流动相为0.1mol·L-1冰醋酸-甲醇(95∶5),流速为1.0mL·mim-1,检测波长为324nm。结果:猫豆胍质量浓度在0.00590～0.0944mg·mL-1范围内与峰面积积分值呈良好的线性关系(r=0.9999);平均回收率为99.92%,RSD=1.68%(n=9)。结论:本方法简便、准确、专属性强、重复性好,可作为猫豆药材或含猫豆胍的药物制剂中猫豆胍的含量测定方法。
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| 关 键 词： | 猫豆 猫豆胍 反相高效液相色谱法 含量测定 |
Determination of Mucunaguanide in Stizolobium cochinchinensis by RP-HPLC |
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| HUANG Zeng-qiong,JIANG Wei-zhe,HUANG Min,HUANG Xing-zhen,WU Shi-hong. Determination of Mucunaguanide in Stizolobium cochinchinensis by RP-HPLC[J]. China Pharmacy, 2010, 0(11): 1000-1001 |
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| Authors: | HUANG Zeng-qiong JIANG Wei-zhe HUANG Min HUANG Xing-zhen WU Shi-hong |
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| Affiliation: | ,HUANG Zeng-qiong(College of Pharmacy, Guangxi Medical University, Nanning 530021, China ;Dept. of Pharmacy, Youjiang Medical College for Nationality, Baise 533000, China) |
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| Abstract: | OBJECTIVE： To establish a RP-HPLC method for the determination of mucunaguanide in Stizolobium cochinchinensis. METHODS： PhenomenexTM C18 column （250 mm×4.6 mm, 5 um） was used.The mobile phase consisted of 0.1 mol.L^-1 glacial acetic acid-methanol （95：5） at a flow rate of 1.0 mL.min^-1,and the detection wavelength was set at 324 nm. RESULTS： The linear range of mucunaguanide were 0.005 90-0.094 4 mg.mL^-1 （r=0.999 9）with average recovery of 99.92% （RSD= 1.68%, n= 9）. CONCLUSION： The method is simple, accurate and specificity with good reproducibility. It can be applied for the content determination of mucunaguanide in S. cochinchinensis or pharmaceutical preparations contained mucunaguanide. |
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| Keywords: | Stizolobium cochinchinensis Mucunaguanide RP-HPLC Content determination |
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