重复经颅磁刺激结合穿梭箱训练对脑梗死恢复期大鼠认知功能的影响

目的:比较重复经颅磁刺激(repetitive transcranial magnetic stimulation, rTMS)单独或与穿梭箱训练结合对短暂性大脑中动脉闭塞(transient middle cerebral artery occlusion,tMCAO)大鼠恢复期认知功能的影响。方法:60只雄性SD大鼠经tMCAO术后,根据神经功能评分分为5组:rTMS刺激组(n=7)、训练组(n=8)、rTMS结合训练组(n=7)、对照组(n=7)、假手术组(n=6),于术后7天开始进行20Hz rTMS和/或穿梭箱训练,术后第1、7、14、21、28天进行改良神经功能缺陷程度评分(mNSS),术后第28天进行Morris水迷宫实验。结果:第28天时rTMS结合训练组(P<0.001)与rTMS刺激组(P<0.05)的mNSS评分均低于对照组。水迷宫实验第1—5天训练组及对照组平均逃避潜伏期较假手术长(P<0.05),第3天rTMS结合训练组的平均逃避潜伏期较rTMS组及训练组短(P<0.05)。对照组穿越平台次数较假手术组少(P<0.05),rTMS结合训练组穿越平台次数较rTMS组、训练组及对照组多(P<0.05)。结论:rTMS结合穿梭箱训练可以改善脑梗死恢复期大鼠的认知功能,效果优于单一的rTMS刺激或训练。

Effects of repetitive transcranial magnetic stimulation combined with shuttle-box training on cognitive function in rats with transient middle cerebral artery occlusion at the recovery stage

Abstract Objective: To compare the effects of repetitive transcranial magnetic stimulation (rTMS) alone or in combination with shuttle-box training on the cognitive function in rats with transient middle cerebral artery occlusion (tMCAO) at the recovery stage. Method: Sixty male Sprague-Dawley rats after tMCAO were divided into 5 groups according to Bederson score: rTMS group (n=7), training group (n=8), rTMS combined with training group (n=7), control group (n=7) and sham group (n=6). 20Hz rTMS and/or shuttle-box training were started on the 7th day after the operation. Each group was assessed with mNSS on day 1,7,14,21,28 and Morris water maze test was carried out on the 56th day after operation. Result: The mNSS scores of the rTMS combined with training group (P<0.001) and the rTMS group (P<0.05) on the 28th day were lower than those of the control group. The escape latency in the training group and control group was longer than that in the sham group at the first to fifth day of the water maze test (P<0.05), and the escape latency in the rTMS combined with training group on the 3rd day was shorter than that in the rTMS group and training group (P<0.05). The times of crossing the platform in the control group were less than that in the sham group (P<0.05), and the times of crossing platform in the rTMS combined with training group were more than that of the rTMS group, training group and control group (P<0.05). Conclusion: rTMS combined with shuttle-box training can improve cognitive function of the tMCAO rats at recovery stage, and the effect is better than rTMS or training alone.