格林巴利综合征相关及单纯腹泻相关空肠弯曲菌细胞毒作用分析

目的分析格林-巴利综合征(GBS)相关空肠弯曲菌细胞毒素(CDT)作用。方法9株GBS相关空肠弯曲菌以及4株腹泻患者分离菌株的全菌蛋白分别以0.001μg～5μg/mL的浓度作用于HeLa细胞,通过观察细胞形态的改变,获得不同菌株对细胞作用的差异。结果不同菌株间产生细胞毒作用的浓度范围从0.01μg/mL到5μg/mL。结论GBS相关菌株对HeLa细胞的细胞毒素作用与腹泻患者分离株相比没有显著差异。不同空肠弯曲菌菌株对HeLa细胞的细胞毒素作用具有菌株特异性。

Cytotoxic effect of Guillain-Barre syndrome-associated and diarrhea-associated Campylobacter jejuni

In order to assess the cytotoxin effect of Campylobacter jejuni（C.jejuni）,the whole cell lysates from 9 strains of Guillan-Barre Syndrome（GBS）-associated C.jejuni and 4 strains of diarrhea-associated C.jejuni were co-cultivated with HeLa cells at the concentration of 0.001-5.00 μg/mL in vitro.The morphologic change of HeLa cells was observed under Olympus BX51 microscope after treatment with different concentration of bacteria lysate in the following 4 days.The morphological changes including swelling,irregularity and lysis of the affected cells over 50% was selected as the cut off for positive change and C.jejuni 81-176 and Helicobacter J99 strains were chosen as the positive and negative control.It was found that the minimum concentration to induce the positive changes in 10 strains（8 GBS associated and 2 strains from diarrhea patients）was 0.1μg/mL and 1 strain with the positive change at the minimum concentration of 1 μg/mL.There was only one GBS-associated strain causing no morphologic change on HeLa cells at the concentration of 5 μg/mL.It was evident that the cytotoxic effect of C.jejuni strains on HeLa cell was strain-specific,and there was no significant difference in the cytotoxic effect on HeLa cell between GBS-associated and diarrhea associated C.jejuni strains.