长期军事训练对氧化应激水平和DNA损伤的影响

目的 探讨长期军事训练对氧化应激水平和DNA损伤的影响.方法 训练组为15名武警战士,对照组为15名普通大学生.两组均进行一次大强度运动应激（5km跑）.训练前后即刻采集静脉血,测定血浆丙二醛（malondialdehyde,MDA）、血浆谷胱甘肽过氧化物酶（glutathione peroxidase,GSH-Px）、血浆肌酸激酶（creatine kinase,CK）和血浆游离DNA（cell-free plasma DNA,cf-DNA）.结果 组间比较,安静状态下,训练组各指标均高于对照组,一次大强度运动后,训练组MDA、CK 和cf-DNA 均低于对照组,而GSH-Px则高于对照组;组内比较,运动前后,对照组和训练组MDA、CK和cf-DNA均显著升高,但训练组上述指标变化幅度明显低于对照组,而GSH-Px无显著性变化.结论 长期军事训练可通过改善机体的氧化应激水平（降低氧化损伤、提高抗氧化能力）并降低细胞DNA损伤从而提高其对急性大强度运动的应激能力.

Effects of Long-term Military Training on Oxidative Stress and DNA Damage

Objective To investigate the effect of long-term military training on oxidative stress and DNA damage. Methods Fifteen soldiers of armed police force （training group） and fifteen normal undergraduate students （control group） both conducted a high-intensity exercise tress （5km running）. Plasma malondialdehyde （MDA）, plasma glutathione peroxidase （GSH-Px）, plasma creatine kinase （CK） and cell-free plasma DNA （cf-DNA） were determined before and after exercise training. Results Though all parameters measured in training group were notably higher than those in control group at rest, MDA, CK and cf-DNA in training group were lower and GSH-Px was higher than control group after a bout of exercise. Both in control group and training group, MDA, CK and cf-DNA were significantly increased after exercise training, but the change extent of the parameters above in training group was significantly lower than those in control group, whereas GSH-Px was not significantly changed. Conclusion Long-term military training may elevated stress ability to high intensity exercise by improving oxidative stress level （reduced oxidative damage and raised antioxidant capacity） and decreasing cell DNA damage.