| 罗布麻叶HPLC指纹图谱构建及在种属鉴别中的应用 |
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| 引用本文： | 韩利文,侯晋军,赵亮,梁泰刚,李青山. 罗布麻叶HPLC指纹图谱构建及在种属鉴别中的应用[J]. 中草药, 2008, 39(4): 591-594 |
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| 作者姓名： | 韩利文 侯晋军 赵亮 梁泰刚 李青山 |
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| 作者单位： | 1. 山西医科大学药学院,山西太原030001;山东省科学院生物研究所,山东济南250014
2. 山西医科大学药学院,山西太原,030001 |
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| 基金项目： | 山西省自然科学基金 , 山西省教育厅攻关基金 , 山西医科大学校科研和教改项目 |
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| 摘 要： | 目的 建立罗布麻叶的HPLC指纹图谱,用以控制罗布麻叶的内在质量,并对罗布麻叶药材与其混淆品进行鉴别.方法 采用HPLC梯度洗脱的方法建立指纹图谱,"计算机辅助相似度评价系统"软件进行数据处理,据此对不同种属的药材进行比较.结果 建立的罗布麻HPLC指纹图谱方法的精密度、稳定性和重现性较好.罗布麻叶(红麻叶)HPLC指纹图谱共标定12个共有指纹峰,与白麻属样品图谱之间呈显著差异,可以依此鉴别.结论 HPLC指纹图谱可以用作罗布麻叶药材的质量控制.
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| 关 键 词： | 罗布麻叶 HPLC 指纹图谱 鉴别 罗布麻叶 HPLC 指纹图谱 种属鉴别 应用 identification application fingerprint 质量控制 差异 样品 白麻 指纹峰 标定 红麻 稳定性 精密度 谱方法 结果 比较 |
| 文章编号： | 0253-2670(2008)04-0591-04 |
| 收稿时间： | 2007-10-01 |
| 修稿时间： | 2007-10-01 |
Establishment of HPLC fingerprint and its application in identification of Folium Apocyni Veneti |
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| HAN Li-wen,HOU Jin-jun,ZHAO Liang,LIANG Tai-gang and LI Qing-shan. Establishment of HPLC fingerprint and its application in identification of Folium Apocyni Veneti[J]. Chinese Traditional and Herbal Drugs, 2008, 39(4): 591-594 |
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| Authors: | HAN Li-wen HOU Jin-jun ZHAO Liang LIANG Tai-gang LI Qing-shan |
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| Affiliation: | School of Pharmaceutical Science,Shanxi Medical University,Taiyuan 030001,China;Institute of Biology,Shandong Academy of Sciences,Jinan 250014,China;School of Pharmaceutical Science,Shanxi Medical University,Taiyuan 030001,China;School of Pharmaceutical Science,Shanxi Medical University,Taiyuan 030001,China;School of Pharmaceutical Science,Shanxi Medical University,Taiyuan 030001,China;School of Pharmaceutical Science,Shanxi Medical University,Taiyuan 030001,China |
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| Abstract: | Objective To establish chromatographic fingerprint of Folium Apocyni Veneti by HPLC and identify Folium Apocyni Veneti from its adulterants.MethodsMethods HPLC Gradient elution was applied to establish the chromatographic fingerprint and"Computer-Aided Similarity Evaluation System"was used in data analyses.Results This chromatographic fingerprint method has good precision,stability,and re- peatability.Twelve common peaks were marked.There are notable differences in chromatographic finger- prints between Folium Apocyni Veneti and its adulterants.Conclusion This chromatographic fingerprint method can be used to evaluate the quality of Folium Apocyni Veneti. |
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| Keywords: | Folium Apocyni Veneti HPLC fingerprint identification |
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