生、炒决明子水提物抗氧化及对一氧化氮、内皮素影响的比较研究

目的:对生、炒决明子水提物抗氧化及舒张血管的作用进行比较,探讨决明子明目的作用机制.方法:小鼠按体重随机分成3组.即正常组,生决明子组,炒决明子组.每组20只,雌、雄各半.给药组每日灌胃给药1次,生药含量10 g·kg~(-1);20 mL·kg~(-1).正常组给相同体积的蒸馏水.连续给药1个月,摘除眼球组织匀浆,取上清液测定超氧化物岐化酶(SOD)、丙二醛(MDA)、乳酸脱氢酶(LDH)及一氧化氮(NO),同时眼眶后静脉丛采血测定内皮素(ET)含量.结果:生、炒决明子水提物有提高小鼠眼组织中SOD和LDH活性、降低MDA含量的作用,以生决明子作用为强.二者均能提高眼组织中NO含量及NO/ET比值,炒决明子作用强于生决明子.结论:生、炒决明子均有一定抗氧化及调节舒张血管作用,决明子明目作用可能是通过此方面来实现.

Comparative study between Cassia obtusifolia and processedCassia obtusifolia on anti-oxidation effect and NO/ET secretion

Objective: To observe the different effects of water soluble extracts from Cassia obtusifolia and processed C. obtusifolia on anti-oxidation and vasodilatation, and to explore the mechanism of eye-clearing effect. Method: Mice were randomly divided into three groups: normal control group, C. obtusifolia group and processed C.obtusifolia group. Ten male and 10 female mice were in each group. mice were given extracts orally 10 g(20 mL) crude drug/kg for one month, control group were given same volume water. Then the eyeball tissue was harvested to prepare homogenate to test SOD, MDA, LDH and NO. Blood was collected from posterior orbital venous plexus to test ET. Result: Both two kinds of extracts could raise the activity of SOD and LDH, decrease the MDA level. Extracts from C.obtusifolia showed higher effects on above mentioned action. Both two kinds of extracts increased the ratio value of NO/ET significantly, and extracts from processed C. obtusifolia showed higher activity. Conclusion: Both C. obtusifolia and processed C. obtusifolia extracts show obvious anti-oxidation and vasodilatation effects. These results may support the eye-clearing activity of C. obtusifolia.