Characteristics of an assay for Rous sarcoma virus and Rous sarcoma cells in tissue culture |
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| Authors: | TEMIN H M RUBIN H |
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| Affiliation: | 1. Centre for Integrative Biology (CBI), Department of Integrated Structural Biology, IGBMC (Institute of Genetics and of Molecular and Cellular Biology), 1 rue Laurent Fries, Illkirch, France;2. Centre National de la Recherche Scientifique (CNRS) UMR 7104, Illkirch, France;3. Institut National de la Santé et de la Recherche Médicale (INSERM) U964, Illkirch, France;4. Université de Strasbourg, Strasbourg, France;5. Laboratoire de Pathologie Comparée, Faculté des Sciences, Université de Montpellier, 34095 Montpellier, France;6. Insect Pest Control Laboratories, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, International Atomic Energy Agency, Vienna, Austria;1. Department of Psychiatry and Neurochemistry, Institute of Neuroscience and Physiology, Sahlgrenska Academy at the University of Gothenburg, 431 80 Mölndal, Sweden;2. Clinical Neurochemistry Laboratory, Sahlgrenska University Hospital, Mölndal, Sweden;3. Department of Molecular Neuroscience, UCL Institute of Neurology, University College London, London, UK;1. Institut für Medizinische Mikrobiologie, Virologie und Hygiene, University Medical Center Eppendorf, Martinistr. 52, 20246 Hamburg, Germany;1. Department of Intensive Care Unit, Affiliated Hospital of Xuzhou Medical University, Xuzhou 221003, China;2. Department of Anesthesiology, Xuzhou Central Hospital, Xuzhou 221009, China;3. Department of Anesthesiology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, 100 Haining Road, Shanghai 200080, China;4. Department of Anesthesiology, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai 200433, China;1. Department of Hygiene, Epidemiology and Medical Statistics, Medical School, National and Kapodistrian University of Athens, Athens, Greece;2. Department of Zoology, University of Oxford, Oxford, UK;3. Department of Epidemiology, Harvard T.H. Chan School of Public Health, Boston, MA, USA |
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| Abstract: | ![]()
An accurate tissue culture assay for Rous sarcoma virus (RSV) and Rous sarcoma cells is described. The Rous sarcoma virus changes a chick fibroblast into a morphologically new and stable cell type with the same chromosomal complement as ordinary chick embryo cells. One virus particle is enough to change one cell, but at any one time 90% of the cells in a culture are not affected by RSV. The cellular resistance is the same in a clonal population. The physiological state of the cell is of some importance in deciding whether or not it is competent to be infected by RSV but so far attempts to infect all the cells in a chick embryo culture by altering the physiological condition have failed. |
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