温病湿热证大鼠模型肝线粒体K+-Na+-ATP酶活力变化的对比研究

目的]探讨多因素复合造模方法对模型大鼠能量代谢的影响。方法]分为正常组、湿热模型组(高脂 高温高湿 大肠杆菌)、模型对照组(普食 高温高湿 大肠杆菌);运用定磷法测肝线粒体Na -K -AT Pase活性。结果]模型组与模型对照组肝线粒体Na -K -AT Pase活性均较正常组显著降低,但两者比较无显著性差异。结论]肝线粒体Na -K -AT Pase活性显著降低,是湿热证模型的病理基础之一,与是否高脂饮食无关。

Comparative Research on Enzyme Activity Change of K+-Na+-ATP of Rats Model with Febrile Damp-heat Syndrome

Objective]To explore the effects of the model establishment methods on energy metabolism indicators of model rats.Methods] Set up normal group,model group(high fat diet high temperature and high humidity colon bacillus),contrary group (routine diet high temperature and high humidity colon bacillus),the activity of liver mitochondria Na -K -ATPase was detected by principle of determines the content of phosphorus.Results] The Na -K -ATPase active inmodel group was remarkably decreased,and had no significantly difference from contrary group.Conclusion]The liver mitochondria Na -K -ATPase being remarkably decreased is one of pathological basis of damp-heat model,un-related with high fat diet.