| Puerarin blocks Na~+ current in rat ventricular myocytes |
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| 引用本文： | ZHANG Guang-Qin,HAO Xue-Mei DAI De-Zai,FU Yu ZHOU Pei-Ai WU Cai-Hong Research Division of Pharmacology,China Pharmaceutical University,Nanjing 210009,China, ~2National Laboratorv of Biomembrane and Membrane Biotechnology,College of Life Sciences,Peking University,Beijing 100871,China. Puerarin blocks Na~+ current in rat ventricular myocytes[J]. Acta pharmacologica Sinica, 2003, 0(12) |
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| 作者姓名： | ZHANG Guang-Qin HAO Xue-Mei DAI De-Zai FU Yu ZHOU Pei-Ai WU Cai-Hong Research Division of Pharmacology China Pharmaceutical University Nanjing 210009 China ~2National Laboratorv of Biomembrane and Membrane Biotechnology College of Life Sciences Peking University Beijing 100871 China |
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| 作者单位： | ZHANG Guang-Qin，HAO Xue-Mei DAI De-Zai，FU Yu ZHOU Pei-Ai WU Cai-Hong Research Division of Pharmacology，China Pharmaceutical University，Nanjing 210009，China; ~2National Laboratorv of Biomembrane and Membrane Biotechnology，College of Life Sciences，Peking University，Beijing 100871，China |
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| 基金项目： | Project supported by the National Natural Science Foundation of China (№ 39970850). |
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| 摘 要： | AIM: To study the effect of puerarin (Pue) on Na~+ channel in rat ventricular myocytes. METHODS: Whole-cell patch-clamp technique was applied on isolated cardiomyocytes from rats. RESULTS: Pue inhibited cardiac I_(Na) in a positive rate-dependent and dose-dependent manner, with an IC_(50) of 349 μmol/L. The kinetics of blockage of cardiac sodium channel by Pue resembled the ClassIa/Ic of antiarrhythmic agents. Pue 300 μmol/L did not alter the shape of the I-V curve of I_(Na), but markedly shifted the steady-state inactivation curve of I_(Na) towards more negative potential by 15.9 mV, and postponed the recovery of I_(Na) inactivation state from (21.9±1.6) ms to (54.4±3.4) ms (P<0.01 ). It demonstrated that the steady state of inactivation was affected by Pue significantly. CONCLUSION: Pue protected ventricular myocytes against cardiac damage and arrhythmias by inhibiting recovery from inactivation of cardiac Na~+ channels.
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Puerarin blocks Na~+ current in rat ventricular myocytes |
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| ZHANG Guang-Qin,HAO Xue-Mei DAI De-Zai,FU Yu ZHOU Pei-Ai WU Cai-Hong Research Division of Pharmacology,China Pharmaceutical University,Nanjing ,China, National Laboratorv of Biomembrane and Membrane Biotechnology,College of Life Sciences,Peking University,Beijing ,China. Puerarin blocks Na~+ current in rat ventricular myocytes[J]. Acta pharmacologica Sinica, 2003, 0(12) |
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| Authors: | ZHANG Guang-Qin HAO Xue-Mei DAI De-Zai FU Yu ZHOU Pei-Ai WU Cai-Hong Research Division of Pharmacology China Pharmaceutical University Nanjing China National Laboratorv of Biomembrane Membrane Biotechnology College of Life Sciences Peking University Beijing China |
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| Affiliation: | ZHANG Guang-Qin,HAO Xue-Mei DAI De-Zai,FU Yu ZHOU Pei-Ai WU Cai-Hong Research Division of Pharmacology,China Pharmaceutical University,Nanjing 210009,China, ~2National Laboratorv of Biomembrane and Membrane Biotechnology,College of Life Sciences,Peking University,Beijing 100871,China |
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| Abstract: | |
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| Keywords: | puerarin myocardium patch-clamp techniques sodium channels |
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