热休克蛋白抑制慢性心理应激引起的焦虑样行为增加

目的：采用HSFI基因敲除小鼠模型探讨热休克蛋白（heat shock protein，HSP）对慢性心理应激引起的焦虑样行为增加的抑制作用。方法：将热休克因子1（heat shock factor 1）基因野生型小鼠（HSFI＋／＋）和热休克因子1基因敲除小鼠（HSF1-／-）暴露于2个月的慢性心理应激，部分小鼠在暴露于慢性心理应激前给予热休克预处理，2个月后采用高架十字迷宫和旷场实验检测各组小鼠焦虑样行为，Western blots检测和比较HSP72，HSC70表达，采用多因素析因设计方差分析对各组小鼠焦虑样行为进行比较。结果：慢性心理应激引起焦虑样行为增加．HSF1基因敲除导致焦虑样行为增加更加明显。热休克预处理使野生型小鼠海马组织中HSP70。HSC70明显升高，并明显减轻了心理应激所致的焦虑样行为的增加。HSF1基因敲除废除了热休克所致的HSP诱导表达及其对心理应激所致焦虑样行为增加的抑制作用。结论：诱导型HSP在抑制心理应激引起的焦虑样行为增加中起着重要的作用。

Use of HSF1-/- Mice to Reveal a Role of HSP in Decreasing Anxiety Caused by Chronic Psychological Stress

Objective: To investigate the potential involvement of HSP in chronic psychological stress-induced anxiety-like behaviour.Methods: HSF1+/+ and HSF1-/-mice were submitted to chronic psychological stress and heat shock pretreatment for 2 months.Anxiety-like behaviour of mice was assessed by elevated-plus maze and open field;expression of HSP72,HSC70 was detected by western blots.3-way ANOVA was used to analyse the anxiety-like behaviour of each group.Results: Chronic psychological stress caused a significant increase in anxiety-like behavior.Heat shock pretreatment increased the expression of HSP72,HSC70 and decreased anxiety-like behavior in HSF1+/+ mice,HSF1 knock out abolished inducible expression of HSP70,HSC70 and their protective effects against chronic psychological stress induced increase of anxiety-like behavior.Conclusion: Inducible heat proteins play an important role in relieving the anxiety-like behaviour induced by chronic psychological stress.