血管紧张素Ⅰ转换酶基因多态性与妊娠高血压综合征的关系

目的:探讨血管紧张素Ⅰ转换酶(ACE)基因多态性与妊娠高血压综合征(妊高征)发病的关系。方法:应用多引物PCR技术检测120例妊高征患者(妊高征组,其中轻度29例、中度31例、重度60例)及110例正常孕妇(对照组)的ACE基因多态性。结果:妊高征组孕妇ACE基因中的II、ID、DD基因型频率分别为20.8%、37.5%、41.7%。对照组孕妇ACE基因中的II、ID、DD基因型频率分别为44.5%、29.1%、26.4%。两组孕妇的DD基因型及D等位基因频率比较,差异有显著性(P<0.05)。妊高征组轻度与重度患者的DD基因型比较,差异有显著性(P<0.05)。回归分析表明,DD基因型及D等位基因与妊高征发病相关。结论:ACE基因中DD基因型是妊高征的易感基因,II基因型是妊高征的保护基因。

Relationships between polymorphism of angiotensin-converting enzyme genes and genetic susceptibility to preeclampsia

Objective To explore the relationships between polymorphism of angiotensin-converting enzyme (ACE) gene and genetic susceptibility to preeclampsia. Methods 120 preeclampsia patients (preeclampsia group), including 29 mild cases, 31 moderate cases and 60 severe cases and 110 normal pregnant women (control group) were recruited. Multiple primers PCR detected the polymorphism of ACE gene. Results In preeclampsia group, the frequencies of genotypes II, ID, and DD of ACE gene were 20.8%, 37.5%and 41.7%respectively. In control group, the frequencies of genotypes II, ID, DD of ACE gene were 44.5%, 29.1%and 26.4%respectively. There existed significant difference between genotype DD and D allele in preeclampsia group and control group(P< 0.05). Compared to mild preeclampsia group, the frequencies of genotypes DD in severe preeclampsia group was significantly higher(P< 0.05). Logistic regression analysis showed that genotypes DD and D allele were associated with preeclampsia. Conclusion Genotype DD may be the risk factor of preeclampsia. Genotype II may have a protective effect against preeclampsia.