高效液相色谱法检测细辛脑注射液的有关物质

【摘要】目的建立细辛脑注射液有关物质的高效液相色谱（HPLC）检测方法。方法采用Diamonsil—C18色谱柱（200mm×4．6mm，5μm）；流动相A为甲醇，流动相B为十二烷基硫酸钠、磷酸二氢钾水溶液，采用梯度洗脱，10min后流动相A在30min内从60％升至80％，维持5min，流速1．0ml／min，检测波长258nm。结果细辛脑的最低检测限量25ng，细辛脑与各杂质分离良好。结论本法简便快速，灵敏度高，准确可靠。

HPLC determination of the related substances of asarone injection

Objective To establish a HPLC method for detection of the asarone injection＇s related substances. Methods The analytical column was Diamonsil - C18 （200 mm × 4.6 mm ,5 μm ）. Using gradient elution method, the mobile phase A was methanol and the mobile phase B was lauryl sodium sulfate, potassium dihydrogen phosphate water so luble fluid （ ten minutes later, the mobile phase A rose from 60% to 80% in thirty -five minutes, kept five minutes ）. The flow rate was 1. 0 ml/min. The detect ion wavelength was 258 nm. Results The detection limits for asarone were 25 ng respectively. Good resolution for asarone and related substances was obtained. Conclusion The method is simple, rapid, sensitive and accurate.